High-fat challenge induced trained innate immunity

Completed

Conditions: atherosclerosis
10003216

Registration Number: NL-OMON47993

Lead Sponsor: Radboud Universitair Medisch Centrum

Brief Summary: Not available

Detailed Description: Not available

Recruitment & Eligibility

Status: Completed

Sex: Not specified

Target Recruitment: 16

Inclusion Criteria

Age between 18 and 40 years
LDL cholesterol < 3.5 mmol/l, fasting triglycerides < 2 mmol/l
No previous cardiovascular events

Exclusion Criteria

Smoking within the year before study entry
Diagnosed with any long-term medical condition that can interfere with the study (i.e. renal failure, galbladder disease, cardiovascular disease, diabetes, rheumatoid arthritis etc.)
Medication (with the exception of oral contraceptives) or supplement use (i.e. omega3)
BMI < 18 or > 27 kg/m2
Previous vaccination within 3 months prior to study entry
Current infection or clinically significant infections within 1 month before study entry (defined as fever > 38.5°C)
Allergic to cow milk/dairy products
Pregnancy/lactation
Abuse of alcohol or drugs
Vegetarian diet

Study & Design

Study Type: Interventional

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>Blood will be drawn at t=0h (before) and at t=1h, t=2h, t=4h, t=6h, t=24 and<br /><br>t=72h after an oral fat load and at the same time points after a control<br /><br>shake. The primary endpoint is the monocyte TNFa production upon ex vivo<br /><br>stimulation with LPS (TLR4 ligand) at t=72h. </p><br>

Secondary Outcome Measures

Name	Time	Method
<p>Additional secondary endpoints are the monocytes* inflammatory phenotype as<br /><br>assessed by flowcytometry analysis , epigenetic and metabolic reprogramming and<br /><br>serum induced inflammation.</p><br>