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Randomized Phase III Study of Intensive Chemotherapy With or Without Dasatinib (Sprycel™)

Phase 3
Completed
Conditions
Acute Myeloid Leukemia (AML)
Interventions
Registration Number
NCT02013648
Lead Sponsor
University of Ulm
Brief Summary

This is a randomized phase III open-label, multicenter trial evaluating standard induction therapy (daunorubicin \[DNR\] and cytarabine \[Ara-C\]) and consolidation therapy (high-dose cytarabine \[HDAC\]) with or without dasatinib in adult patients with newly diagnosed CBF-AML

Detailed Description

This is a randomized phase III open-label, multicenter trial evaluating standard induction therapy (daunorubicin \[DNR\] and cytarabine \[Ara-C\]) and consolidation therapy (high-dose cytarabine \[HDAC\]) with or without dasatinib in adult patients with newly diagnosed CBF-AML; in the investigational arm, consolidation therapy is followed by a one-year maintenance therapy with dasatinib. Patients with molecular disease persistence or molecular relapse as assessed by quantitative RQ-PCR for the CBF fusion transcripts will be eligible for hematopoietic stem cell transplantation before overt hematologic relapse occurs. Primary endpoint is event-free survival.

AML patients will be assessed for the CBF fusion genes in one of two AMLSG central laboratories within 48 hours of diagnosis, and only patients with CBF-AML will be enrolled.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
204
Inclusion Criteria
  • Core-binding factor (CBF) AML with molecular diagnosis of RUNX1-RUNX1T1 fusion transcript resulting from t(8;21)(q22;q22.1) (or a variant form) or of CBFB-MYH11 fusion transcript resulting from inv(16)(p13.1q22)/t(16;16)(p13.1;q22) as assessed in one of the central AMLSG reference laboratories (Ulm, Hannover)
  • Age ≥ 18; there is no upper age limit
  • No prior chemotherapy for leukemia except hydroxyurea for up to 5 days during the diagnostic screening phase
  • Non-pregnant and non-nursing. Due to the unknown teratogenic potential of dasatinib in humans, pregnant or nursing patients may not be enrolled. Women of childbearing potential (WOCBP) must have a negative serum or urine pregnancy test within a sensitivity of at least 25 mIU/mL with-in 72 hours prior to registration. Women of child-bearing potential must either commit to continued abstinence from heterosexual intercourse or begin TWO acceptable methods of birth control - one highly effective method (e.g., IUD, hormonal, tubal ligation, or partner's vasectomy), and one additional effective method (e.g., latex condom, diaphragm, or cervical cap) - AT THE SAME TIME, at least four weeks before she begins dasatinib therapy. "Women of childbearing potential" is defined as a sexually active mature woman who has not undergone a hysterectomy or who has had menses at any time in the preceding 24 consecutive months.
  • Men must agree not to father a child and must use a latex condom during any sexual contact with women of childbearing potential while taking dasatinib and for 3 months after therapy is stopped, even if they have undergone a successful vasectomy.
  • Signed written informed consent.
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Exclusion Criteria
  • Performance status WHO >2
  • Pulmonary edema and/or pleural/pericardial effusion within 14 days of day 1. If edema/effusion resolves to CTC Grade ≤1, patients can be treated with dasatinib.
  • Patients with ejection fraction <50% by echocardiography within 14 days of day 1
  • Organ insufficiency (creatinine >1.5x upper normal serum level; bilirubin, AST or AP >2.5x upper normal serum level; heart failure NYHA III/IV; severe obstructive or restrictive ventilation disorder)
  • Uncontrolled infection
  • Patients with a "currently active" second malignancy other than non-melanoma skin cancers. Patients are not considered to have a "currently active" malignancy, if they have completed therapy and are considered by their physician to be at less than 30% risk of relapse within one year.
  • Severe neurological or psychiatric disorder interfering with ability of giving an informed consent
  • Known positive for HIV, active HBV, HCV, or Hepatitis A infection
  • Bleeding disorder independent of leukemia
  • No consent for registration, storage and processing of the individual disease characteristics and course as well as information of the family physician and/or other physicians involved in the treatment of the patient about study participation.
  • No consent for biobanking.
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Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Standard armCytarabinePatients will receive induction therapy with daunorubicin 60 mg/m2/day administered on days 1-3 (when daunorubicin is not available due to supply shortage: Idarubicin 12mg²/day on days 1,3,5) and cytarabine 200 mg/m2/day administered by continuous IV infusion on days 1-7. Patients achieving PR only at the end of cycle 1 will receive a second induction cycle with daunorubicin 50 mg/m2/day (when daunorubicin is not available due to supply shortage: Idarubicin 10 mg²/day on days 1 and 3) administered on days 1-3 and cytarabine 200 mg/m2/day administered by cont. IV infusion daily on days 1-5. Patients will receive 4 cycles of consolidation therapy. Consolidation therapy consists of high-dose cytarabine 3 g/m2 (\>60 years: 1 g/m2) q12h, days 1-3 administered intravenously over three hours. Follow-up period: There is no maintenance therapy in the standard arm. Patients will be closely followed, in particular for molecular disease persistence or molecular relapse.
Standard armDaunorubicinPatients will receive induction therapy with daunorubicin 60 mg/m2/day administered on days 1-3 (when daunorubicin is not available due to supply shortage: Idarubicin 12mg²/day on days 1,3,5) and cytarabine 200 mg/m2/day administered by continuous IV infusion on days 1-7. Patients achieving PR only at the end of cycle 1 will receive a second induction cycle with daunorubicin 50 mg/m2/day (when daunorubicin is not available due to supply shortage: Idarubicin 10 mg²/day on days 1 and 3) administered on days 1-3 and cytarabine 200 mg/m2/day administered by cont. IV infusion daily on days 1-5. Patients will receive 4 cycles of consolidation therapy. Consolidation therapy consists of high-dose cytarabine 3 g/m2 (\>60 years: 1 g/m2) q12h, days 1-3 administered intravenously over three hours. Follow-up period: There is no maintenance therapy in the standard arm. Patients will be closely followed, in particular for molecular disease persistence or molecular relapse.
Standard armIdarubicinPatients will receive induction therapy with daunorubicin 60 mg/m2/day administered on days 1-3 (when daunorubicin is not available due to supply shortage: Idarubicin 12mg²/day on days 1,3,5) and cytarabine 200 mg/m2/day administered by continuous IV infusion on days 1-7. Patients achieving PR only at the end of cycle 1 will receive a second induction cycle with daunorubicin 50 mg/m2/day (when daunorubicin is not available due to supply shortage: Idarubicin 10 mg²/day on days 1 and 3) administered on days 1-3 and cytarabine 200 mg/m2/day administered by cont. IV infusion daily on days 1-5. Patients will receive 4 cycles of consolidation therapy. Consolidation therapy consists of high-dose cytarabine 3 g/m2 (\>60 years: 1 g/m2) q12h, days 1-3 administered intravenously over three hours. Follow-up period: There is no maintenance therapy in the standard arm. Patients will be closely followed, in particular for molecular disease persistence or molecular relapse.
Investigational armDasatinibPatients will receive induction therapy with daunorubicin 60 mg/m2/day on days 1-3 (when daunorubicin is not available due to supply shortage: Idarubicin 12mg²/day on days 1,3,5) and cytarabine 200 mg/m2/day by cont. IV infusion on days 1-7. Patients will receive dasatinib 100 mg QD on days 8-21. Patients achieving PR only at the end of cycle 1 will receive a 2nd induction cycle with daunorubicin 50 mg/m2/day on days 1-3 (when daunorubicin is not available due to supply shortage: Idarubicin 10 mg²/day on days 1 and 3) and cytarabine 200 mg/m2/day by cont. IV infusion on days 1-5. Patients will receive dasatinib 100 mg QD on days 6-21. Consolidation therapy (4 cycles). Treatment consists of high-dose cytarabine 3 g/m2 (\>60 years: 1 g/m2) q12h, days 1-3 iv over 3 hours. Patients will receive dasatinib 100 mg QD on days 4-21. Maintenance therapy: Patients completing consolidation therapy will continue to receive single agent dasatinib 100 mg QD for one year (or until relapse).
Investigational armCytarabinePatients will receive induction therapy with daunorubicin 60 mg/m2/day on days 1-3 (when daunorubicin is not available due to supply shortage: Idarubicin 12mg²/day on days 1,3,5) and cytarabine 200 mg/m2/day by cont. IV infusion on days 1-7. Patients will receive dasatinib 100 mg QD on days 8-21. Patients achieving PR only at the end of cycle 1 will receive a 2nd induction cycle with daunorubicin 50 mg/m2/day on days 1-3 (when daunorubicin is not available due to supply shortage: Idarubicin 10 mg²/day on days 1 and 3) and cytarabine 200 mg/m2/day by cont. IV infusion on days 1-5. Patients will receive dasatinib 100 mg QD on days 6-21. Consolidation therapy (4 cycles). Treatment consists of high-dose cytarabine 3 g/m2 (\>60 years: 1 g/m2) q12h, days 1-3 iv over 3 hours. Patients will receive dasatinib 100 mg QD on days 4-21. Maintenance therapy: Patients completing consolidation therapy will continue to receive single agent dasatinib 100 mg QD for one year (or until relapse).
Investigational armIdarubicinPatients will receive induction therapy with daunorubicin 60 mg/m2/day on days 1-3 (when daunorubicin is not available due to supply shortage: Idarubicin 12mg²/day on days 1,3,5) and cytarabine 200 mg/m2/day by cont. IV infusion on days 1-7. Patients will receive dasatinib 100 mg QD on days 8-21. Patients achieving PR only at the end of cycle 1 will receive a 2nd induction cycle with daunorubicin 50 mg/m2/day on days 1-3 (when daunorubicin is not available due to supply shortage: Idarubicin 10 mg²/day on days 1 and 3) and cytarabine 200 mg/m2/day by cont. IV infusion on days 1-5. Patients will receive dasatinib 100 mg QD on days 6-21. Consolidation therapy (4 cycles). Treatment consists of high-dose cytarabine 3 g/m2 (\>60 years: 1 g/m2) q12h, days 1-3 iv over 3 hours. Patients will receive dasatinib 100 mg QD on days 4-21. Maintenance therapy: Patients completing consolidation therapy will continue to receive single agent dasatinib 100 mg QD for one year (or until relapse).
Investigational armDaunorubicinPatients will receive induction therapy with daunorubicin 60 mg/m2/day on days 1-3 (when daunorubicin is not available due to supply shortage: Idarubicin 12mg²/day on days 1,3,5) and cytarabine 200 mg/m2/day by cont. IV infusion on days 1-7. Patients will receive dasatinib 100 mg QD on days 8-21. Patients achieving PR only at the end of cycle 1 will receive a 2nd induction cycle with daunorubicin 50 mg/m2/day on days 1-3 (when daunorubicin is not available due to supply shortage: Idarubicin 10 mg²/day on days 1 and 3) and cytarabine 200 mg/m2/day by cont. IV infusion on days 1-5. Patients will receive dasatinib 100 mg QD on days 6-21. Consolidation therapy (4 cycles). Treatment consists of high-dose cytarabine 3 g/m2 (\>60 years: 1 g/m2) q12h, days 1-3 iv over 3 hours. Patients will receive dasatinib 100 mg QD on days 4-21. Maintenance therapy: Patients completing consolidation therapy will continue to receive single agent dasatinib 100 mg QD for one year (or until relapse).
Primary Outcome Measures
NameTimeMethod
Event-free Survival4 years

To assess event-free survival (EFS) after intensive induction (daunorubicin and cytarabine) and consolidation (high-dose cytarabine) chemotherapy with or without dasatinib in patients with CBF-AML

Secondary Outcome Measures
NameTimeMethod
overall survival4 years
relapse-free survival4 years
Cumulative incidence of relapse (CIR)4 years
Cumulative incidence of death (CID)4 years
PIA analysis4 years

Pharmacodynamic inhibition of KIT as assessed by the KIT plasma inhibitory assay (PIA)

toxicity7 months (standard arm) / 19 months (investigational arm)

Type, frequency, severity (graded using the National Cancer Institute Common Terminology Criteria for Adverse Events \[NCI CTCAE\] version 4.03), timing and relatedness of non-hematologic toxicity observed during different treatment cycles.

Trial Locations

Locations (54)

Hanuschkrankenhaus

🇦🇹

Wien, Austria

Krankenhaus der Barmherzigen Schwestern

🇦🇹

Linz, Austria

Universitätsklinik der PMU

🇦🇹

Salzburg, Austria

Knappschaftskrankenhaus Bochum

🇩🇪

Bochum, Germany

Universitätsklinikum Schleswig-Holstein

🇩🇪

Kiel, Schleswig-Holstein, Germany

MVZ Osthessen

🇩🇪

Fulda, Hessen, Germany

Helios Klinikum Bad Saarow, Klinik für Hämatologie

🇩🇪

Bad Saarow, Germany

Charité Universitätsmedizin Campus Virchow Klinikum

🇩🇪

Berlin, Germany

St. Johannes Hospital

🇩🇪

Dortmund, Germany

Klinikum Aschaffenburg-Alzenau

🇩🇪

Aschaffenburg, Germany

Klinikum am Urban

🇩🇪

Berlin, Germany

Klinikum Neukölln

🇩🇪

Berlin, Germany

Malteser Krankenhaus St. Franziskus-Hospital

🇩🇪

Flensburg, Germany

Universitätsklinikum Medizinische Klinik und Poliklinik III

🇩🇪

Bonn, Germany

Klinikum Bremen Mitte gGmbH

🇩🇪

Bremen, Germany

Universitätsklinikum Medizinische Klinik und Poliklinik

🇩🇪

Düsseldorf, Germany

Klinikum Darmstadt Medizinische Klinik V

🇩🇪

Darmstadt, Germany

Klinikum Esslingen

🇩🇪

Esslingen, Germany

Universitätsklinikum Freiburg

🇩🇪

Freiburg, Germany

Wilhelm-Anton-Hospital gGmbH

🇩🇪

Goch, Germany

Klinikum Region Hannover GmbH

🇩🇪

Hannover, Germany

Ortenau Klinikum

🇩🇪

Offenburg, Germany

Marienhospital Klinikum der Ruhr-Universität

🇩🇪

Herne, Germany

Märkische Kliniken GmbH

🇩🇪

Lüdenscheid, Germany

III. Medizinische Klinik und Poliklinik Universitätsmedizin der Johannes Gutenberg-Universität

🇩🇪

Mainz, Germany

Johannes Wesling Klinikum

🇩🇪

Minden, Germany

Klinikum Mutterhaus der Borromäerinnen gGmbH

🇩🇪

Trier, Germany

Medizinische Universitätsklinik

🇩🇪

Tübingen, Germany

Stauferklinikum Schwäbisch Gmünd

🇩🇪

Mutlangen, Germany

PIUS Hospital

🇩🇪

Oldenburg, Germany

Klinikum rechts der Isar der TU

🇩🇪

München, Germany

Klinikum Oldenburg gGmbH

🇩🇪

Oldenburg, Germany

Klinikum Passau

🇩🇪

Passau, Germany

Caritasklinkum Saarbrücken St. Theresia

🇩🇪

Saarbrücken, Germany

Schwarzwald-Baar Klinikum

🇩🇪

Villingen Schwenningen, Germany

Kliniken Essen Süd

🇩🇪

Werden, Germany

HELIOS Klinikum

🇩🇪

Wuppertal, Germany

Medizinische Hochschule Hannover

🇩🇪

Hannover, Germany

Städtisches Klinikum Braunschweig gGmbH

🇩🇪

Braunschweig, Germany

Universitätsmedizin Göttingen

🇩🇪

Göttingen, Germany

Universitätsklinikum des Saarlandes

🇩🇪

Homburg, Germany

Städtisches Klinikum Karlsruhe gGmbH

🇩🇪

Karlsruhe, Germany

Gemeinschaftspraxis Hämato-Onkologie

🇩🇪

Lebach, Germany

Univ-Klinikum der Otto-von Guericke-Universität

🇩🇪

Magdeburg, Germany

Universitätsklinikum Ulm Zentrum für Innere Medizin

🇩🇪

Ulm, Germany

Universitätsklinik für Innere Medizin V

🇦🇹

Innsbruck, Austria

Universitätsklinikum Hamburg-Eppendorf

🇩🇪

Hamburg, Germany

Klinikum Hanau GmbH

🇩🇪

Hanau, Germany

SLK-Kliniken GmbH

🇩🇪

Heilbronn, Germany

Klinikum Lippe GmbH

🇩🇪

Lemgo, Germany

Klinikum Stuttgart

🇩🇪

Stuttgart, Germany

Universitätsklinikum Regensburg

🇩🇪

Regensburg, Germany

Vinzenz von Paul Kliniken gGmbH Marienhospital

🇩🇪

Stuttgart, Germany

Krankenhaus der Elisabethinen Linz GmbH

🇦🇹

Linz, Austria

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