A pilot study about culturing laryngeal tumor tissue organoids to facilitate new treatment strategies for laryngeal cancer in the future
Recruiting
- Conditions
- Laryngeal cancerLaryngeal carcinoma10027656
- Registration Number
- NL-OMON55128
- Lead Sponsor
- niversitair Medisch Centrum Groningen
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Recruiting
- Sex
- Not specified
- Target Recruitment
- 20
Inclusion Criteria
- proven carcinoma of the larynx.
- > 18 yrs of age
- planned routine biopsy or planned surgical resection as part of standard
diagnostic work-up or treatment
- expected tumor volume > 2 cm3
- informed consent
Exclusion Criteria
- no squamous cell carcinoma after definitive histolopathological analysis
Study & Design
- Study Type
- Observational non invasive
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method <p>In ex vivo LC organoids: viable and proliferating organoids after at least 2<br /><br>and 6 weeks in 50% of the samples.<br /><br>To assess viability and to confirm the origin of each tumor specimen, the<br /><br>specimen will be evaluated by analysis of non-necrotic tumor tissue in<br /><br>hematoxylin-eosin stained tissue sections and by determining proliferation rate<br /><br>on day 0, week 2 and week 6, using organoid formation and growth rate and by<br /><br>using whole genome DNA sequencing (~30x coverage (~100 Gb)). </p><br>
- Secondary Outcome Measures
Name Time Method <p>We will analyse differences in solidity of ex vivo LC organoids between primary<br /><br>and recurrent LC tissue by using a Chi-square test with SPSS. </p><br>