Association of Single Nucleotide Polymorphisms (SNPs) in Metalloproteinases (MMPs) Genes With the Risk of Primary Knee Osteoarthritis in Greek Population.

• Conditions: Metalloproteinase SNPs -1575 G/A MMP-2, 836 A/G MMP-9 and -77 A/G MMP-13 and Risk for Developing Knee Osteoarthritis in Greek Population
• Interventions: Other: No intervention, Patients selection based on clinical symptoms and radiological imaging

• Registration Number: NCT04463290
• Lead Sponsor: Aristotle University Of Thessaloniki

Brief Summary

Does the presence of gene polymorphisms -1575G / A in the MMP-2 gene, 836A / G in the MMP-9 gene and -77A / G in the MMP-13 gene, affect the risk of developing idiopathic knee osteoarthritis in the Greek population?

Detailed Description

This is a prospective case-control study in which the patients' genotype will be studied against the genotype of the controls. The study is prospective and not retrospective, as data will be collected from patients with primary knee osteoarthritis as well as the control group from the outset. The study intends to include 200 people. The first group consists of 100 individuals suffering from knee osteoarthritis, while the second group will include 100 healthy controls without osteoarthritis. From each one DNA will be isolated and analyzed from collected blood. The prospective study allows valid data to be collected on patient demographics and disease severity. Due to the large number of cases of the disease, it is estimated that the necessary specimens will be collected within a reasonable time from the beginning of this thesis. The choice of the patient-control study is necessary to limit the influence of confounding factors (age, gender, obesity, etc.). This type of study includes rigorous selection of patients and controls with specific entry and exclusion criteria, selection of controls based on matching patient characteristics, stratification, and subgroup analyzes. The final result will be a comparison of the incidence of specific gene polymorphisms between patients with knee osteoarthritis and the normal population.

Study Timeline

• Study First Submitted: 2020-07-05
• Study First Submitted QC: 2020-07-05
• Study Start: 2020-07-06
• Study First Posted: 2020-07-09
• Status Verified: 2022-08
• Last Update Submitted: 2022-08-17
• Last Update Posted: 2022-08-18
• Primary Completion: 2022-11-28
• Study Completion: 2022-12-30

Recruitment & Eligibility

• Status: UNKNOWN
• Sex: All
• Target Recruitment: 200

Inclusion Criteria

• Patients with primary knee osteoarthritis are selected based on clinical symptoms and radiological imaging with a score at least 2 on the Kellgren & Lawrence scale.
• Patients without symptomatology or radiographic image of knee osteoarthritis (score <2 on Kellgren & Lawrence scale) will be selected as controls.

Exclusion Criteria

• systemic inflammatory disease
• rheumatic disease
• liver disease
• renal failure
• neoplasm or other chronic disease

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
Knee Osteoarthritis Patients Group	No intervention, Patients selection based on clinical symptoms and radiological imaging	Patients suffering from primary knee osteoarthritis
Control Group	No intervention, Patients selection based on clinical symptoms and radiological imaging	Healthy people without suffering from primary knee osteoarthritis

Primary Outcome Measures

Name	Time	Method
The frequency of presence of gene polymorphisms -1575G / A MMP-2 gene, 836A / G MMP-9 gene and -77A / G MMP-13 gene, in Greek patients with knee osteoarthritis comparing with healthy people	6 months

Trial Locations

Locations (1)

3rd Orthopedic Department , Pagageorgiou Hospital; 🇬🇷 Thessaloníki, Athens, Greece