Evaluation of Bacterial Flora Among Operation Theatre Staff at a Tertiary Care Hospital

Completed

• Conditions: Microbial Colonization
• Interventions: Other: Swab stick

• Registration Number: NCT04089683
• Lead Sponsor: Kamineni Academy of Medical Sciences and Research Centre

Brief Summary

Health care associated infection (HCAI) is a serious health hazard as it leads to increased morbidity and mortality of patients, length of hospital stay and costs associated with increased hospital stay.

A total of 361 subjects divided into four groups were included in this study. The first group comprised of 179 doctors, the second had 31 nurses, third group had 110 OT Technicians and the fourth group included 41 subjects which included housekeeping staff and cleaners. Swabs were collected from OT staff at the time of entry and at exit from the OT. Places of swabs taken were: A) Web space, B) OT dress and C) Anterior nares.

Detailed Description

Introduction:

Health care associated infection (HCAI) is a serious health hazard as it leads to increased morbidity and mortality of patients, length of hospital stay and costs associated with increased hospital stay. Infections from OT is well known and is well established which are often referred to as "hot zones" for emergence \& spread of microbial resistance. Contamination in an operation theatre is an important cause of HCAIs and is the third leading cause of HCAIs.

This was a prospective observational study conducted in the operation theatre of a tertiary hospital once a month randomly from April 2014 - April 2016. All Operation theatre (OT) staff were included in the study. All the patients in OT and samples from the OT air \& equipment were excluded from the study. A total of 361 subjects divided into four groups were included in this study. The first group comprised of 179 doctors, the second had 31 nurses, third group had 110 OT Technicians and the fourth group included 41 subjects which included housekeeping staff and cleaners. Swabs were collected from OT staff at the time of entry and at exit from the OT. Places of swabs taken were: A) Web space, B) OT dress and C) Anterior nares.

Study Timeline

• Study Start: 2014-04
• Primary Completion: 2016-04
• Study Completion: 2016-04
• Status Verified: 2019-09
• Study First Submitted: 2019-09-06
• Study First Submitted QC: 2019-09-12
• Last Update Submitted: 2019-09-12
• Study First Posted: 2019-09-13
• Last Update Posted: 2019-09-13

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 361

Inclusion Criteria

All Operation Theatre staff -

Exclusion Criteria

Patients in Operation Theatre, Operating room air & equipment

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
Doctors	Swab stick	Swabs collected from Web space, Scrub, Anterior nares before entering Operation theatre and while exit from operation theatre
Nurses	Swab stick	Swabs collected from Web space, Scrub, Anterior nares before entering Operation theatre and while exit from operation theatre
OT Technicians	Swab stick	Swabs collected from Web space, Scrub, Anterior nares before entering Operation theatre and while exit from operation theatre
House keeping staff and cleaners	Swab stick	Swabs collected from Web space, Scrub, Anterior nares before entering Operation theatre and while exit from operation theatre

Primary Outcome Measures

Name	Time	Method
Bacterial load on OT dress among OT staff	2 years	Swabs will be taken from OT dress while donning them and before going out of OT at the end of the day and cultured to look for growth
To identify the nasal carriers of organisms among OT staff	2 years	Swabs will be taken from anterior nares while entering OT at the end of the day while leaving OT and cultured to look for growth
Bacterial flora harboured at Web spaces among OT staff	2 years	Swabs will be taken from web spaces before entering Operation Theatre and while going out of OT at the end of the day and cultured to look for growth of Bacteria