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The INTER-CARDIO Study: The Impact of Commonly Consumed Fat on Cardiovascular Health; a Randomised Controlled Trial

Not Applicable
Completed
Conditions
Healthy Adults
Interventions
Dietary Supplement: Butter
Dietary Supplement: Rapeseed oil
Dietary Supplement: Interesterified fat
Dietary Supplement: Non- interesterified fat
Registration Number
NCT03438084
Lead Sponsor
King's College London
Brief Summary

The purpose of this study is to investigate whether there are differences in postprandial cardiometabolic metabolic indices following interesterified fats used commercially versus application appropriate non- interesterifed alternative fat.

Detailed Description

Aim: The current study aims to investigate the acute effects of commercially available interesterified fat versus versus application appropriate non- interesterifed alternative fat on 8 h postprandial cardiometabolic outcomes.

Hypothesis: Interesterified fat will induce a different cardiomtabolic response compared to a non interesterifed, application appropriate fat.

Subjects: Participants will include 40 healthy male and female volunteers aged between the ages of 35 and 75 years (since during this age metabolic changes start to take place).

Power calculation: Based on previous studies carried out by our group at King's College London, a sample size of 20 participants (males and females separately) will allow a 2.16% (percentage unit) difference in FMD to be detected (90% power, 0.05 alpha, SD 2.9. 50 participants will be recruited allowing for a 20% dropout rate.

Expected value:The study will provide novel information on the acute effects of commercially relevant processed fats on postprandial lipaemia.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
50
Inclusion Criteria
  • Males and females aged 35-75 years
  • Healthy (free of diagnosed diseases listed in exclusion criteria)
  • Able to understand the information sheet and willing to comply with study protocol
  • Able to give informed consent
Exclusion Criteria
  • Medical history of myocardial infarction, angina, thrombosis, stroke, cancer, liver or bowel disease or diabetes
  • Body mass index < 20 kg/m2 or > 35 kg/m2
  • Plasma cholesterol ≥7.5 mmol/L
  • Plasma triacylglycerol > 3 mmol/L
  • Plasma glucose > 7 mmol/L
  • Blood pressure ≥140/90 mmHg
  • Current use of antihypertensive or lipid lowering medications
  • Alcohol intake exceeding a moderate intake (> 28 units per week)
  • Current cigarette smoker (or quit within the last 6 months)
  • ≥ 20% 10-year risk of CVD as calculated using a risk calculator

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Arm && Interventions
GroupInterventionDescription
Saturated fat controlButterButter. 50g fat
ControlRapeseed oilRapeseed oil. 50 g fat.
InteresterifiedInteresterified fatCommercially available interesterifed fat spread. 50g fat.
Non- interesterifiedNon- interesterified fatCommercially available non-interesterified fat. 50g fat.
Primary Outcome Measures
NameTimeMethod
Endothelial functionUp to 8 hours

Flow mediated dilatation

Postprandial lipaemic responseUp to 8 hours

Area under plasma TAG concentration/ time curve

Secondary Outcome Measures
NameTimeMethod
Postprandial lipaemic responseUp to 8 hours

Postprandial plamsa total fatty acid composition and non esterifed fatty acid

Metabolomic for assessment of atherogenic potential (lipoprotein) analysisUp to 8 hours

Lipid concentrations and compositions of 14 lipoprotein subclasses, measured by NMR

Glycaemic responseUp to 8 hours

Plasma glucose concentration

Insulinaemic responseUp to 8 hours

Plasma insulin concnetration

Nitric oxide bioavailabilityUp to 8 hours

Plasma nitric oxide species and NADPH oxidase activity

Inflammatory responseUp to 8 hours

Plasma IL-6

Oxidative stressUp to 8 hours

Plasma 8-isoprostane F2a

Trial Locations

Locations (2)

King's College London

🇬🇧

London, United Kingdom

Department of Nutritional Sciences, Faculty of Life Sciences and Medicine, Kings College London

🇬🇧

London, United Kingdom

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