Anorectal Function and Neuronal-glial-epithelial Unit in Healthy Subjects
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Healthy
- Sponsor
- Rennes University Hospital
- Enrollment
- 16
- Locations
- 1
- Primary Endpoint
- Number of enteric neurons per ganglion
- Last Updated
- 6 years ago
Overview
Brief Summary
This study aims to assess the phenotype of the enteric nervous system (enteric neurons) in healthy subjects.
The enteric nervous system (ENS) is composed by enteric neurons and enteric glial cells. There is a cross-talk between ENS and the intestinal epithelial barrier (IEB). ENS and IEB together constitute the neuronal-glial-epithelial unit. This unit has a key role in gut functions. The characteristics and the phenotype of the ENS also change according to age and environmental factors. Similar study is ongoing for patients with Spina Bifida. Anorectal data and phenotype of the neuronal-glial-epithelial unit in healthy subjects is required to assess abnormality of these items in patients with neurological disease including Spina Bifida.
Investigators
Eligibility Criteria
Inclusion Criteria
- •subject aged between 18 and 65 years old age and sex matched with the first sixteen patients included and evaluated in the ANOSPIN study
- •subject who gave their written consent to participate to the present study
- •subject not included in another study
- •subject covered by social insurance system
Exclusion Criteria
- •Pregnant or nursing women;
- •Anticoagulants therapy or coagulation disorders;
- •Past history of anal or rectal surgery
- •Crohn disease or ulcerative colitis
- •Irritable bowel syndrome according to the Rome III criteria;
- •Past history of pelvic radiotherapy
- •Pas history of rectal or anal cancer
- •Anorectal symptoms : faecal incontinence, constipation, anorectal pain, rectal prolapse;
- •CCIS \>5 (faecal incontinence);
- •Kess Score \>9 (constipation);
Outcomes
Primary Outcomes
Number of enteric neurons per ganglion
Time Frame: data collected at the day of endoscopy
colonic biopsies will be used to study intestinal permeability, colonic inflammation and enteric nervous system
Secondary Outcomes
- density and activity of the enteric glial cells(data collected at the day of endoscopy)
- rectal adaptation(data collected at the day of endoscopy)
- rectal perception(data collected at the day of endoscopy)
- anal distensiblity(data collected at the day of endoscopy)
- intestinal permeability(data collected at the day of endoscopy)
- macroscopic and microscopic colonic inflammation(data collected at the day of endoscopy)