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Influence of Rifampin Co-Administration on the Pharmacokinetic Profile of Daptomycin

Early Phase 1
Completed
Conditions
Drug Interactions
Pharmacokinetics
Interventions
Registration Number
NCT02097953
Lead Sponsor
University of Utah
Brief Summary

The purpose of this study is to understand how an antibiotic, rifampin, may change the blood levels of another antibiotic, daptomycin, in the body. In addition, the effect of polymorphisms in P-glycoprotein (a protein involved in the removal of daptomycin from the body) on the blood levels of daptomycin will be evaluated. The hypotheses are that rifampin will decrease the blood levels of daptomycin and that the effect will be greater for certain P-glycoprotein polymorphisms.

Detailed Description

This will be an open-label, sequential pharmacokinetic study in 12 healthy volunteers. After providing informed consent, participants meeting inclusion criteria will be administered daptomycin 6 mg/kg over 2 minutes and blood and urine samples will be collected over a 24 hour period after infusion. Following this, participants will be given a 13 day supply of rifampin 600 mg/day to be taken at home. After completion of the rifampin course, a second pharmacokinetic sampling will be performed (day 15 overall). Participants will be administered a dose of rifampin 600 mg then once again be administered daptomycin 6 mg/kg over 2 minutes and blood and urine samples will be collected over a second 24 hour period. A blood sample will be obtained at the first study visit for determination of each participant's P-glycoprotein genotype.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
12
Inclusion Criteria
  • Ability to provide informed consent
  • If female of child bearing potential, on reliable method of contraception and negative pregnancy test at enrollment
Exclusion Criteria
  • Obesity (body mass index > 30)
  • Creatinine clearance < 80 ml/min
  • Creatine phosphokinase > 2 times upper limit of normal at enrollment
  • Aspartate aminotransferase, alanine aminotransferase, or alkaline phosphatase > 3 times upper limit of normal at enrollment
  • History of liver disease or cirrhosis
  • History of congestive heart failure
  • Allergy or intolerance to rifampin or daptomycin
  • Need for concomitant non-study medications during the study period
  • Inability to abstain from grapefruit juice or herbal supplements (such as St. John's wort) during the study period
  • Receipt of study drugs within 30 days prior to enrollment
  • Pregnancy or lactation
  • Inability to adhere to study procedures

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
Daptomycin and RifampinDaptomycinDrug: Daptomycin Daptomycin 6 mg/kg will be given by IV infusion on the first study day Drug: Rifampin Rifampin 600 mg capsules will be given orally once daily for 14 days starting on study day 2 Drug: Daptomycin Daptomycin 6 mg/kg will be given by IV infusion on study day 15
Daptomycin and RifampinRifampinDrug: Daptomycin Daptomycin 6 mg/kg will be given by IV infusion on the first study day Drug: Rifampin Rifampin 600 mg capsules will be given orally once daily for 14 days starting on study day 2 Drug: Daptomycin Daptomycin 6 mg/kg will be given by IV infusion on study day 15
Primary Outcome Measures
NameTimeMethod
Daptomycin Free and Total Peak Concentrations14 days

The free and total peak concentrations of daptomycin before and after administration of rifampin

Daptomycin Free and Total 24 hour Concentrations14 days

The free and total 24 hour concentrations of daptomycin before and after administration of rifampin

Daptomycin Free and Total Area Under the Concentration-Time Curve (AUC) from 0 to 24 hours14 days

The free and total AUC from 0 to 24 hours of daptomycin before and after administration of rifampin

Daptomycin Free and Total AUC from 0 to Infinity14 days

The free and total AUC from 0 to infinity of daptomycin before and after administration of rifampin

Percent Protein Binding of Daptomycin14 days

The percent protein binding of daptomycin before and after administration of rifampin

Daptomycin total, renal, and non-renal clearance14 days

Total, renal, and non-renal clearance of daptomycin before and after administration of rifampin

Daptomycin Volume of Distribution14 days

The volume of distribution of daptomycin before and after administration of rifampin

Daptomycin Elimination Rate Constant14 days

The elimination rate constant of daptomycin before and after administration of rifampin

Secondary Outcome Measures
NameTimeMethod
P-glycoprotein Polymorphism14 days

The effect of different P-glycoprotein polymorphisms on the pharmacokinetic profile of daptomycin before and after administration of rifampin will be assessed

Number of Participants with Adverse Events45 days

Safety monitoring will be done by regular adverse event assessment, laboratory and vital sign monitoring, and physical exam.

Trial Locations

Locations (1)

University of Utah

🇺🇸

Salt Lake City, Utah, United States

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