T cells in Nose of Older adults (TINO)

Recruiting

Conditions: Respiratory infections, Aging

Registration Number: NL-OMON24946

Lead Sponsor: MC

Brief Summary: Not available

Detailed Description: Not available

Recruitment & Eligibility

Status: Recruiting

Sex: Not specified

Target Recruitment: 170

Inclusion Criteria

• Healthy elderly (>65yrs) that are not frail (frailty score 1-3)
• Frailty (frailty score =4) elderly (>65yrs) These will be consist of individuals without a history of recurrent respiratory infections or with >2 self-reported episodes of respiratory infection in the past year.
• Healthy young (18-35yrs) adults

Exclusion Criteria

• Incompetence to provide informed consent prior or during study
• Current smoker or >40 pack year history
• History of severe nose bleedings
• Diagnosed with asthma, COPD or chronic rhinosinusitis
• Use of inhalation corticosteroids or antibiotics in the past 6 weeks
• Current use of anti-coagulants (to prevent nosebleeds)
• Respiratory tract infection or common cold in the past 2 weeks
• Immunocompromised individuals (with primary immune deficiency or secondary immune deficiency)
• Life expectancy <28 days in the opinion of study physician
• Vaccination in the 2 months prior to study start

A potential subject that is only excluded from participation based on a recent vaccination will be asked to re-participate 2 months post vaccination.

Study & Design

Study Type: Observational non invasive

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
To compare nasal CD8+ T cell frequency between young adults and frail older adults.

Secondary Outcome Measures

Name	Time	Method
1. In depth profiling of T cells in nose and blood of young adults and older adults with and without frailty.<br>2. Assess the stability of T cell populations and other immune populations over time.<br>3. Compare blood and nasal T cells between older adults with and without recurrent respiratory tract infections.<br>4. Compare other nasal and systemic immune populations and parameters between young adults, vital older adults and frail older adults (with or without recurrent infections).<br>5. Associate nasal and systemic factors (e.g. cytokines and metabolites) and with T cells.<br>6. Associate respiratory tract microbiota with T cells and other immune parameters.<br>7. Associate covariates, such as biological age, HLA type and sex with T cells and other immune parameters.<br>8. Assess the impact of acute respiratory tract infection on (antigen-specific) T cell populations and other immune parameters in nose and blood.