Clinical and Biochemical Evaluation of Platelets Rich Fibrin (PRF) and Nanohydroxyapatite in the Treatment of Intrabony Defects
Overview
- Phase
- Early Phase 1
- Intervention
- OFD alone
- Conditions
- Bony Defects
- Sponsor
- King Abdulaziz University
- Enrollment
- 60
- Locations
- 1
- Primary Endpoint
- Changes in Gingival crevicular fluid (GCF)
- Status
- Completed
- Last Updated
- 2 years ago
Overview
Brief Summary
Background: the ultimate goal of periodontal therapy is the regeneration of periodontal tissues. Researches had demonstrated the benefits of platelet concentrates bine grafts in osseous regeneration in the areas of periodontal surgery.
Aim of the study: to evaluate the effect of PRF with/without nanohydroxyapatite in periodontal intrabony defects.
Detailed Description
Background: the ultimate goal of periodontal therapy is the regeneration of periodontal tissues. The regenerative and wound healing potential of platelets has attracted much attention over the last few years. Researches had demonstrated the benefits of platelet concentrates in osseous regeneration in the areas of periodontal surgery and implantology. In addition, nanohydroxyapatite was shown to be unsheathed by newly formed bone and partly replaced by it, but resorbed very slowly, as shown in animal experiments after 9 months or in clinical studies after a 6-month observation period. The clinical and biochemical studies evaluating the regenerative effect of PRF alone or in combination with nanohydroxyapatite in periodontal intrabony defects are still deficient. Aim of the study: to evaluate clinical and biochemical effect of PRF with/without nanohydroxyapatite in treatment of human intrabony defects.
Investigators
Maha A. Bahammam
Associate Professor and Consultant of Periodontology
King Abdulaziz University
Eligibility Criteria
Inclusion Criteria
- •All patients are free from systemic illness.
- •All patients should be diagnosed as having chronic periodontitis.
- •Patients should be cooperative, motivated and willing to follow our treatment protocol and follow up visits.
- •Patients selected had not received antibiotics or anti-inflammatory therapy in the 6 months prior to examination.
Exclusion Criteria
- •Presence of areas of periodontal pocket of (probing depth ≥5 and clinical attachment level (CAL) ≥ 3).
- •Patients who have received any type of periodontal treatment in the past 6 months prior to examination.
Arms & Interventions
OFD alone
Control group: including 15 defects that will receive open flap debridement (OFD).
Intervention: OFD alone
PRF + OFD
Test group (1): including 15 defects that will receive platelet rich fibrin (PRF) with open flap debridement (OFD).
Intervention: OFD alone
PRF + OFD
Test group (1): including 15 defects that will receive platelet rich fibrin (PRF) with open flap debridement (OFD).
Intervention: Platelet rich Fibrin
Bone + OFD
Test group (2): including 15 defects that will receive nanohydroxyapatite bone graft with open flap debridement OFD).
Intervention: OFD alone
Bone + OFD
Test group (2): including 15 defects that will receive nanohydroxyapatite bone graft with open flap debridement OFD).
Intervention: Nanohydroxyapatite
PRF + Bone + OFD
Test group (3): including 15 defects that will receive nanohydroxyapatite bone graft with platelet rich fibrin (PRF) after open flap debridement (OFD).
Intervention: OFD alone
PRF + Bone + OFD
Test group (3): including 15 defects that will receive nanohydroxyapatite bone graft with platelet rich fibrin (PRF) after open flap debridement (OFD).
Intervention: Platelet rich Fibrin
PRF + Bone + OFD
Test group (3): including 15 defects that will receive nanohydroxyapatite bone graft with platelet rich fibrin (PRF) after open flap debridement (OFD).
Intervention: Nanohydroxyapatite
Outcomes
Primary Outcomes
Changes in Gingival crevicular fluid (GCF)
Time Frame: up to 3 weeks.
GCF will be collected after removing saliva and supra-gingival plaque using methylcellulose filter paper strip that will be inserted in the periodontal pocket and will be let in for 30 seconds.
Secondary Outcomes
- Changes in Gingival index(up to 3 months)
- Changes in level of attachment loss(up to 3 months)
- Changes in probing pocket depth(up to 3 months)