Effect of fat on levels of fat-sensitive sensors in the small bowel and their relationship with gut function and body weight in healthy lean and obese humans.

Not Applicable

Completed

• Conditions: Obesity; Healthy human physiology; Diet and Nutrition - Obesity; Oral and Gastrointestinal - Normal oral and gastrointestinal development and function

• Registration Number: ACTRN12612000376842
• Lead Sponsor: Dr Tanya Little

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Results First Posted: 1900-01-01
• Study Start: 2012-04-03
• Last Update Posted: 13 January 2020

Recruitment & Eligibility

• Status: Completed
• Sex: All
• Target Recruitment: 60

Inclusion Criteria

Thirty healthy lean (BMI: 19-25 kg/m2), and 30 obese (BMI: >30 kg/m2, waist circumference: males >100 cm; females >97 cm) Caucasian volunteers aged 18-55 years will be recruited. Participants will be age- and sex-matched, weight-stable (<5 % fluctuation) and not on a weight-loss diet for a minimum of 3 months before inclusion. Pre-menopausal female participants will habitually take a hormonal contraceptive. This will eliminate the effects of the menstrual cycle on GI function and energy intake.

Exclusion Criteria

-significant gastrointestinal symptoms; disease or surgery
-eating disorders
-current use of any prescribed or non-prescribed medications which affect energy metabolism, GI function, body weight or appetite
-history of any bleeding disorder, or use of anticoagulant or antiplatelet drugs (e.g. warfarin, aspirin, clopidogrel)
-diabetes mellitus (fasting blood glucose > 6.9 mmol/l and/or glycated haemoglobin > 6.2 %)
-epilepsy
-cardiovascular or respiratory disease
-any other significant illness
-allergy to local anaesthetic
-intake of > 20 g alcohol on a daily basis
-smokers (cigarettes, cigars, marijuana)
-use of fish oil
-restrained eaters, as determined by a score of > 12 on the eating restraint component of the Three Factor Eating Questionnaire (eating restraint will be assessed in all participants, but only used as an exclusion criterion in lean participants, as in our experience, the obese will have some degree of eating restraint)
-donation of blood in the 12 weeks prior to enrolment in the study. Participants will also be instructed to abstain from donating blood for 12 weeks after study completion. -A screening blood sample will be taken to ensure that only individuals with normal haemoglobin, iron and ferritin levels are included in the study.
-participation in any other research studies in the 12 weeks prior to enrolment in the study
-history of taste disorders
-consumption of a vegetarian diet
-high performance athletes
-inability to comprehend study protocol

Study & Design

• Study Type: Interventional
• Study Design: Not specified