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Acute Physiological Effects of Greek Traditional Dancing

Not Applicable
Recruiting
Conditions
Menopause
Muscle Damage
Body Composition
Physical Performance
Cardiovascular Health
Interventions
Other: Slow tempo
Other: Moderate tempo
Other: Fast tempo
Registration Number
NCT06260124
Lead Sponsor
University of Thessaly
Brief Summary

In Greece, people of different age groups, including young children to older adults, are involved in traditional dance. To date, the well-know benefits of dancing include entertainment, socialization and increased physical activity. However, the acute effects of Greek traditional dancing on health, physical performance and muscle damage indices remain largely unknown. Therefore, the aim of this project is to evaluate the acute effect of Greek traditional dancing on health-, physical performance-, and muscle damage-related parameters by considering the impact of dancing tempo (slow vs moderate vs fast). In a crossover repeated measures design 10 pre- and 10 post-menopausal women will participate in the three dancing sessions of different tempo in a random order.

Detailed Description

This study aims at determining the acute effect of Greek traditional dancing on health- and physical performance-related parameters by considering the impact of dancing tempo. Ten pre- and ten postmenopausal women meeting the inclusion criteria will be assigned to a crossover trial. Initially, participants will undergo baseline testing including (i) anthropometrics, (ii) body composition, (iii) physical performance, (iv) physical activity level and (v) dietary intake. After baseline measurements, a 7-day familiarization period will take place, during which participants will practice daily on the three experimental dances. Each dance will be characterized by a different tempo (beats per minute) so that there will be one dance from each tempo category (i.e. slow, moderate and fast). After familiarization, participants will execute in a random order the three dancing sessions, on separate days. Each dancing session will consist of one dance (of either slow or moderate or fast tempo) lasting \~ 3- 4 minutes. During each dance heart rate, oxygen consumption and activity intensity will be continuously monitored. Before and immediately after the dance systolic and diastolic blood pressure, perceived exertion and blood lactate will be measure. In addition, before each dance and at 24 and 48 hours post-dance muscle soreness, muscle strength and resting metabolic rate will be measured and a resting blood sample will be drawn for the assessment of inflammatory and oxidative stress markers.

Recruitment & Eligibility

Status
RECRUITING
Sex
Female
Target Recruitment
20
Inclusion Criteria
  • Premenopausal and postmenopausal women.
  • Abstain from vigorous physical activity and/or exercise during the last (at least) 6 months prior to the study.
  • Free of musculoskeletal diseases and injuries.
  • Free of cardiometabolic diseases.
  • Free of mental health disorders
  • No body weight loss >10% over the last 6 months prior to the study
  • Non-smokers
Exclusion Criteria
  • Perimenopause and menopause women
  • Participation vigorous physical activity and/or exercise during the last (at least) 6 months prior to the study
  • Presence of musculoskeletal diseases and injuries.
  • Presence of cardiometabolic diseases.
  • Presence of mental health disorders
  • Body weight loss >10% over the last 6 months prior to the study
  • Smokers

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Arm && Interventions
GroupInterventionDescription
Post-menopausalModerate tempoPost-menopausal healthy, inactive women that will participate in a random order in three dancing sessions (Greek traditional dancing) of different tempo on non-consecutive days.
Post-menopausalFast tempoPost-menopausal healthy, inactive women that will participate in a random order in three dancing sessions (Greek traditional dancing) of different tempo on non-consecutive days.
Post-menopausalSlow tempoPost-menopausal healthy, inactive women that will participate in a random order in three dancing sessions (Greek traditional dancing) of different tempo on non-consecutive days.
Pre-menopausal womenSlow tempoPre-menopausal healthy, inactive women that will participate in a random order in three dancing sessions (Greek traditional dancing) of different tempo on non-consecutive days.
Pre-menopausal womenFast tempoPre-menopausal healthy, inactive women that will participate in a random order in three dancing sessions (Greek traditional dancing) of different tempo on non-consecutive days.
Pre-menopausal womenModerate tempoPre-menopausal healthy, inactive women that will participate in a random order in three dancing sessions (Greek traditional dancing) of different tempo on non-consecutive days.
Primary Outcome Measures
NameTimeMethod
Change in delayed-onset of muscle soreness (DOMS)At baseline and at 24 hours and 48 hours after the dance

DOMS of the knee extensors and flexors of both limbs will be evaluated by palpation of the relaxed muscle's belly and distal region with participants rating the perceived soreness on a visual analogue scale ranging from 1 to 10.

Change in lymphocytesAt baseline and at 24 hours and 48 hours after the dance

Lymphocytes will be measured using an automatic blood analyzer

Change in catalase activityAt baseline and at 24 hours and 48 hours after the dance

Catalase activity will be measured spectrophotometrically in red blood cells

Change in total antioxidant capacityAt baseline and at 24 hours and 48 hours after the dance

Total antioxidant capacity will be measured spectrophotometrically in plasma

Change in oxygen consumptionThrough dance session completion, an average of 30 minutes

Oxygen consumption will be continuously monitored using a portable metabolic system.

Change in maximal isometric voluntary contractionAt baseline and at 24 hours and 48 hours after the dance

Maximal isometric voluntary contraction of the knee extensors and flexors of both limbs will be assessed using an isokinetic dynamometer.

Change in granulocyte countAt baseline and at 24 hours and 48 hours after the dance

Granulocyte count will be measured using an automatic blood analyzer

Change in monocytesAt baseline and at 24 hours and 48 hours after the dance

Monocytes will be measured using an automatic blood analyzer

Change in blood lactateAt baseline and immediately after the dance

Blood lactate will be measured on a portable, automatic lactate analyser using the relevant strips

Change in resting metabolic rateAt baseline and at 24 hours and 48 hours after the dance

Resting metabolic rate (kcal) will be assessed using a portable open-circuit indirect calorimeter with a ventilated hood system

Change in joint range of motionAt baseline and at 24 hours and 48 hours after the dance

Knee joint range of motion will be assessed using a manual goniometer

Change in white blood cell countAt baseline and at 24 hours and 48 hours after the dance

White blood cell count will be measured using an automatic blood analyzer

Change in reduced glutathioneAt baseline and at 24 hours and 48 hours after the dance

Reduced glutathione will be measured spectrophotometrically in red blood cells

Change in heart rateThrough dance session completion, an average of 30 minutes

Heart rate will be continuously monitored using heart rate sensors

Change in blood pressureAt baseline and immediately after the dance

Systolic and diastolic blood pressure will be measured using a blood pressure cuff

Change in perceived exertionAt baseline and immediately after the dance

Perceived exertion will be rated using the Borg Rating of Perceived Exertion scale, a numerical scale that ranges from 6 (no exertion) to 20 (maximum effort).

Change in functional performanceAt baseline and at 24 hours and 48 hours after the dance

Functional performance will be assessed using the movement-based screening tool functional movement screen (FMS)

Change in protein carbonylsAt baseline and at 24 hours and 48 hours after the dance

Protein carbonyls will be measured spectrophotometrically in red blood cells

Change in physical activityThrough dance session completion, an average of 30 minutes

The number of steps performed will be assessed using an accelerometer (ActiGraph Gt3x)

Change in creatine kinase concentrationAt baseline and at 24 hours and 48 hours after the dance

Creatine kinase will be measured using an automatic blood analyzer and commercially available kits

Change in oxidized glutathioneAt baseline and at 24 hours and 48 hours after the dance

Oxidized glutathione will be measured spectrophotometrically in red blood cells

Secondary Outcome Measures
NameTimeMethod
Change in hematocritAt baseline and at 24 hours and 48 hours after the dance

Hematocrit will be measured using an automatic blood analyzer

Change in red blood cell countAt baseline and at 24 hours and 48 hours after the dance

Red blood cell count will be measured using an automatic blood analyzer

Change in hemoglobinAt baseline and at 24 hours and 48 hours after the dance

Hemoglobin will be measured using an automatic blood analyzer

Trial Locations

Locations (1)

University of Thessaly, Department of Physical Education and Sport Science

🇬🇷

Tríkala, Thessaly, Greece

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