An Intervention Study of Sustainable Food Habits That Promotes Health and Environmental Aspects
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Sustainability
- Sponsor
- Mälardalen University
- Enrollment
- 39
- Locations
- 1
- Primary Endpoint
- Changes in concentration of pesticide residues in urine
- Status
- Completed
- Last Updated
- 7 years ago
Overview
Brief Summary
The aim of this study is to investigate changes in nutrient intake, the human gut microbiota and pesticide excretion in urine when shifting from conventional food habits to sustainable food habits.
Investigators
Eligibility Criteria
Inclusion Criteria
- •Living in Västerås
- •Carnivores
Exclusion Criteria
- Not provided
Outcomes
Primary Outcomes
Changes in concentration of pesticide residues in urine
Time Frame: Baseline, after 4 weeks, after 8 weeks
The investigators will assess changes in concentration of four pesticide residues (Mepiquat, Chlormequat, Propamocarb and Ethylene thiourea) excreted in urine. Samples will be collected at three occasions; baseline (reference sample), midtime (after 4 weeks) and at the end (after 8 weeks). The concentration of pesticide residues in urine samples will be determined in normal or reversed phase configuration on a high performance liquid chromatograph coupled to a triple quadrupole mass spectrometer (HPLC-MS/MS) system with electrospray ionization interface (ESI) run in negative or positive mode.
Changes in composition of the microbiota
Time Frame: Baseline, after 4 weeks, after 8 weeks
The investigators will assess changes over time in the presence of all gut bacteria species in the microbiome of the participants based on DNA sequencing from fecal samples. Samples will be collected at three occasions; baseline (reference sample), midtime (after 4 weeks) and at the end (after 8 weeks). From frozen samples, all DNA will be extracted and full shotgun metagenomic sequencing performed using next generation sequencing technologies (Illumina and BGISEQ). A total of 120 samples (3\*40 participants) will be sequenced to a depth of at least 3 giga base pairs (Gbp) each.
Secondary Outcomes
- Changes in intake of zinc(Baseline, after 4 weeks, after 8 weeks)
- Changes in intake of selenium(Baseline, after 4 weeks, after 8 weeks)
- Changes in intake of fat(Baseline, after 4 weeks, after 8 weeks)
- Changes in intake of thiamin(Baseline, after 4 weeks, after 8 weeks)
- Changes in intake of riboflavin(Baseline, after 4 weeks, after 8 weeks)
- Changes in intake of carbohydrates(Baseline, after 4 weeks, after 8 weeks)
- Changes in intake of vitamin E(Baseline, after 4 weeks, after 8 weeks)
- Changes in intake of niacin(Baseline, after 4 weeks, after 8 weeks)
- Changes in intake of vitamin B6(Baseline, after 4 weeks, after 8 weeks)
- Changes in intake of iodine(Baseline, after 4 weeks, after 8 weeks)
- Changes in intake of energy(After 8 weeks)
- Changes in intake of protein(Baseline, after 4 weeks, after 8 weeks)
- Changes in intake of dietary fiber(Baseline, after 4 weeks, after 8 weeks)
- Changes in intake of vitamin A(Baseline, after 4 weeks, after 8 weeks)
- Changes in intake of vitamin D(Baseline, after 4 weeks, after 8 weeks)
- Changes in intake of folate(Baseline, after 4 weeks, after 8 weeks)
- Changes in intake of vitamin C(Baseline, after 4 weeks, after 8 weeks)
- Changes in intake of iron(Baseline, after 4 weeks, after 8 weeks)
- Changes in intake of calcium(Baseline, after 4 weeks, after 8 weeks)
- Changes in intake of phosphorus(Baseline, after 4 weeks, after 8 weeks)
- Changes in intake of vitamin B12(Baseline, after 4 weeks, after 8 weeks)
- Changes in intake of potassium(Baseline, after 4 weeks, after 8 weeks)
- Changes in intake of copper(Baseline, after 4 weeks, after 8 weeks)
- Assessment of the participants likeability of the diets using a questionnaire(After 8 weeks)