Effect of Oily Fish Intake, Sex, Age, BMI, and APOE Genotype on Plasma Long Chain n-3 Fatty Acid Composition

Completed

• Conditions: Plasma Omega-3 Status

• Registration Number: NCT03245749
• Lead Sponsor: University of Southampton

Brief Summary

The purpose of this study is to investigate the effect of habitual oily fish intake, sex, age, BMI, and APOE genotype on plasma long chain n-3 fatty acid composition.

Detailed Description

The marine long chain n-3 polyunsaturated fatty acids (LC n-3 PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are beneficial to cardiovascular and cognitive health and the concentration of EPA and DHA present in blood and tissue lipids is positively correlated with these effects.

As a result, national and global authorities recommend regular consumption of oily fish in order to provide approximately 500 mg EPA+DHA per day in a healthy population. However, the associations between EPA and DHA intake and blood and tissue status, and therefore physiological benefits, are highly variable and the factors influencing this heterogeneity are not well understood.

This study aims to investigate the effect of habitual oily fish intake as well as sex, age, BMI, and Apolipoprotein E (APOE) genotype on LC n-3 PUFA status in plasma phosphatidylcholine, non-esterified fatty acids, cholesteryl esters and triacylglycerols in order to obtain greater knowledge of the determinants of LC n-3 PUFA status with the potential to develop more robust, and perhaps subgroup specific, recommendations for EPA and DHA intake.

Study Timeline

• Study Start: 2003-06
• Primary Completion: 2005-09
• Study Completion: 2006-03
• Study First Submitted: 2017-08-07
• Study First Submitted QC: 2017-08-07
• Study First Posted: 2017-08-10
• Status Verified: 2021-07
• Last Update Submitted: 2021-07-04
• Last Update Posted: 2021-07-08

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 374

Inclusion Criteria

Not provided

Exclusion Criteria

• Diagnosed endocrine dysfunction including diabetes
• Fasting glucose concentration > 6.5 mmol/L
• Myocardial infarction in the previous 2 years
• The use of medication that may interfere with lipid metabolism
• Fasting total cholesterol of > 8.0 mmol/L
• TG of > 3.0 mmol/L
• Currently following a weight loss diet
• Taking n-3 fatty acid supplements

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Plasma long chain n-3 polyunsaturated fatty acid concentrations	Baseline	Concentrations of eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA) and docosahexaenoic acid (DHA) in plasma phosphatidylcholine, non-esterified fatty acids, cholesteryl esters and triacylglycerols

Secondary Outcome Measures

Name	Time	Method
APOE genotyping	Baseline	APOE genotype determined from blood sample
Anthropometric measurements	Baseline	Height and weight to determine BMI

Trial Locations

Locations (5)

Newcastle University; 🇬🇧 Newcastle upon Tyne, United Kingdom

University of Glasgow; 🇬🇧 Glasgow, United Kingdom

University of Southampton; 🇬🇧 Southampton, United Kingdom

Univeristy of East Anglia; 🇬🇧 Norwich, United Kingdom

University of Reading; 🇬🇧 Reading, United Kingdom