Matrix Metalloproteinases's in Oral Biofluids During Pregnancy and Postpartum

Completed

• Conditions: Pregnancy
• Interventions: Other: Saliva collection; Other: GCF collection

• Registration Number: NCT03516565
• Lead Sponsor: Aydin Adnan Menderes University

Brief Summary

The aim of this study was to investigate salivary and gingival crevicular fluid (GCF) levels of MMP-8, MMP-9 and TIMP-1 during 2/3 trimester of pregnancy and after delivery

Detailed Description

MMP-8 and MMP-9 have been reported to reflect the inflammatory status in periodontal tissues and, therefore represent as potential biomarkers of periodontitis. The balance between tissue inhibitors of metalloproteinases (TIMPs) and MMPs play an important role in maintaining the integrity of healthy periodontium.

Study Timeline

• Study Start: 2013-04-01
• Primary Completion: 2014-10-15
• Study Completion: 2015-09-01
• Status Verified: 2018-04
• Study First Submitted: 2018-04-24
• Study First Submitted QC: 2018-04-24
• Last Update Submitted: 2018-04-24
• Study First Posted: 2018-05-04
• Last Update Posted: 2018-05-04

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Female
• Target Recruitment: 114

Inclusion Criteria

• Pregnant group included women, who were in their second trimester (weeks 16-24) and third trimester (weeks 25-34)

Exclusion Criteria

• (a) any known systemic disease, (b) periodontal treatment within the last six months, (c) patients having less than 10 teeth, (d) smokers and (e) individuals with BMI > 30 kg/m2

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
Non-pregnant group	Saliva collection	Women who were systemically healthy and non-pregnant.
Postpartum group	Saliva collection	Postpartum group included women, who were evaluated 6 months after giving birth
Pregnancy group	Saliva collection	Pregnant group included women, who were in their second trimester (weeks 16-24) and third trimester (weeks 25-34)
Postpartum group	GCF collection	Postpartum group included women, who were evaluated 6 months after giving birth
Non-pregnant group	GCF collection	Women who were systemically healthy and non-pregnant.
Pregnancy group	GCF collection	Pregnant group included women, who were in their second trimester (weeks 16-24) and third trimester (weeks 25-34)

Primary Outcome Measures

Name	Time	Method
Changing MMP-9 levels	6 months	Salivary and GCF level measured by ELISA
Changing TIMP-1 levels	6 months	Salivary and GCF level measured by ELISA
Changing MMP-8 levels	6 months	Salivary and GCF level measured by IFMA