Influence of Protein Hydrolysis on Dietary Protein Digestibility and Metabolism in Healthy Subjects

Not Applicable

Completed

• Conditions: Healthy
• Interventions: Other: Intestinal and metabolic exploration; Dietary Supplement: mixture of AA; Dietary Supplement: 15N-labelled intact casein; Dietary Supplement: 15N-labelled hydrolyzed casein

• Registration Number: NCT00873951
• Lead Sponsor: Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement

Brief Summary

Hydrolysis of dietary protein may impact their gastrointestinal kinetics and further metabolism. The primary goal of this work was to measure the metabolic fate of intact or hydrolyzed protein using a protein of good nutritional quality, i.e., casein. A second aim of this study was to assess the true ileal digestibility of dietary protein using different methods including the hydrolyzed casein method and to measure its possible effect on endogenous intestinal N losses in humans.

Detailed Description

Not available

Study Timeline

• Study Start: 2006-01
• Primary Completion: 2006-11
• Study Completion: 2008-01
• Status Verified: 2009-03
• Study First Submitted: 2009-04-01
• Study First Submitted QC: 2009-04-01
• Last Update Submitted: 2009-04-01
• Study First Posted: 2009-04-02
• Last Update Posted: 2009-04-02

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 26

Inclusion Criteria

• healthy subjects
• BMI between 18 and 25

Exclusion Criteria

• allergy to dairy proteins
• positive serology to AgHbS, HCV or HIV
• pregnancy
• abusive drug or alcohol consumption.

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
AA	mixture of AA	Subjects undergo an intestinal and metabolic exploration following the ingestion of a standard mixed meal containing 15% of energy as a mixture of AA mimicking the composition of casein but devoid in serine
Intact casein	Intestinal and metabolic exploration	Subjects undergo an intestinal and metabolic exploration following the ingestion of a standard mixed meal containing 15% of energy as 15N-labelled intact casein
Intact casein	15N-labelled intact casein	Subjects undergo an intestinal and metabolic exploration following the ingestion of a standard mixed meal containing 15% of energy as 15N-labelled intact casein
Hydrolyzed casein	Intestinal and metabolic exploration	Subjects undergo an intestinal and metabolic exploration following the ingestion of a standard mixed meal containing 15% of energy as 15N-labelled hydrolyzed casein
Hydrolyzed casein	15N-labelled hydrolyzed casein	Subjects undergo an intestinal and metabolic exploration following the ingestion of a standard mixed meal containing 15% of energy as 15N-labelled hydrolyzed casein
AA	Intestinal and metabolic exploration	Subjects undergo an intestinal and metabolic exploration following the ingestion of a standard mixed meal containing 15% of energy as a mixture of AA mimicking the composition of casein but devoid in serine

Primary Outcome Measures

Name	Time	Method
Postprandial metabolic fate of dietary N	0h-1h-2h-3h-4h-5h-6h-7h-8h postprandial

Secondary Outcome Measures

Name	Time	Method
Differences in ileal digestibility determination according to the method used	8h postprandial

Trial Locations

Locations (1)

Centre de recherche sur volontaires; 🇫🇷 Bobigny, France