Understanding the Relationship Between Inflammation and Insulin Resistance in the Adipose Tissue

Not Applicable

Completed

• Conditions: Obesity; Insulin Resistance
• Interventions: Procedure: Fat biopsy during scheduled abdominal surgery; Procedure: Fat Aspiration; Dietary Supplement: Fish Oil Supplement

• Registration Number: NCT02378077
• Lead Sponsor: Mayo Clinic

Brief Summary

This study is being done to better understand the relationship between inflammation and insulin resistance in your Adipose (fat) Tissue .

Detailed Description

This study is being done to determine whether eosinophils (a type of white blood cell) play an important role in human fat metabolism and inflammation.

This study is being done to determine if fish oil supplement could play a role in reducing inflammation and improving insulin sensitivity in your fat tissue.

Study Timeline

• Study Start: 2014-08
• Study First Submitted: 2014-09-17
• Study First Submitted QC: 2015-02-26
• Study First Posted: 2015-03-04
• Primary Completion: 2018-10
• Study Completion: 2018-10
• Status Verified: 2020-01
• Last Update Submitted: 2020-01-14
• Last Update Posted: 2020-01-18

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 28

Inclusion Criteria

Not provided

Exclusion Criteria

Not provided

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
Lean or Obese, Non-Diabetic	Fat biopsy during scheduled abdominal surgery	To determine whether eosinophil content of adipose tissue is related to insulin sensitivity. We will use euglycemic clamps, fat biopsy (obtained during a scheduled abdominal surgery) and fat aspiration for analysis of subcutaneous (Sc) and omental (OM) adipose tissue from obese, insulin resistant and lean, insulin sensitive volunteers to test the hypothesis that, as in mice, eosinophil content in human subcutaneous and omental white adipose tissue, inversely correlates with body weight, with skeletal muscle and hepatic insulin sensitivity.
Fish oil supplementation	Fat Aspiration	Determine whether, in adipose tissue, levels of, anti-inflammatory molecules correlate with insulin sensitivity and whether these levels are altered by a treatment designed to promote resolution of inflammation. Volunteers will take a fish oil supplement for three months.
Lean or Obese, Non-Diabetic	Fat Aspiration	To determine whether eosinophil content of adipose tissue is related to insulin sensitivity. We will use euglycemic clamps, fat biopsy (obtained during a scheduled abdominal surgery) and fat aspiration for analysis of subcutaneous (Sc) and omental (OM) adipose tissue from obese, insulin resistant and lean, insulin sensitive volunteers to test the hypothesis that, as in mice, eosinophil content in human subcutaneous and omental white adipose tissue, inversely correlates with body weight, with skeletal muscle and hepatic insulin sensitivity.
Fish oil supplementation	Fish Oil Supplement	Determine whether, in adipose tissue, levels of, anti-inflammatory molecules correlate with insulin sensitivity and whether these levels are altered by a treatment designed to promote resolution of inflammation. Volunteers will take a fish oil supplement for three months.

Primary Outcome Measures

Name	Time	Method
Eosinophil content in adipose tissue	baseline	Eosinophils number will be measured by flow cytometry in subcutaneous and visceral fat of lean vs obese, non-diabetic volunteers

Secondary Outcome Measures

Name	Time	Method
Relationship between eosinophils content and body mass index (BMI)	baseline	Measure as a linear regression between BMI (kg/m2) and eosinophils number in the fat

Trial Locations

Locations (1)

Mayo Clinic in Arizona; 🇺🇸 Scottsdale, Arizona, United States