Investigating the Effect of Obesity on Eosinophil Recruitment in Human Adipose Tissue

Not Applicable

Completed

• Conditions: Obese; Healthy
• Interventions: Procedure: Fat Tissue Biopsy

• Registration Number: NCT04234295
• Lead Sponsor: Mayo Clinic

Brief Summary

Researchers are trying to better understand the relationship between inflammation in adipose tissue (AT) and insulin resistance.

Detailed Description

Not available

Study Timeline

• Study First Submitted: 2020-01-15
• Study First Submitted QC: 2020-01-15
• Study First Posted: 2020-01-21
• Study Start: 2020-01-29
• Status Verified: 2022-01
• Primary Completion: 2022-01-17
• Study Completion: 2022-01-17
• Last Update Submitted: 2022-01-17
• Last Update Posted: 2022-01-19

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 24

Inclusion Criteria

• Adults ages 30-50 years
• Non-smokers
• Lean subjects with a BMI of 25 or less, gender specific normal body fat,
• Obese, non-diabetic subjects with a BMI between 30 and 50 and taking no medications affecting glucose metabolism or lipid metabolism
• subjects scheduled for an elective laparoscopic or laparotomy abdominal surgery

Exclusion Criteria

• Receiving corticosteroid therapy or have a history of asthma, COPD or atopic syndrome
• taking any biologic treatment for any autoimmune disorder
• Taking any leukotriene receptor antagonist acting medications (ex. Montelukast)
• smoking

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Lean, Healthy Controls	Fat Tissue Biopsy	Subjects with a body mass index (BMI) of 25 or less will have a fat tissue biopsy collected
Obese, Non-Diabetic	Fat Tissue Biopsy	Subject with a BMI between 30 and 50 kg/m2 will have fat tissue biopsy collected

Primary Outcome Measures

Name	Time	Method
Number of eosinophils in adipose tissue	baseline	eosinophil number will be measured by flow cytometry in subcutaneous (superficial) adipose tissue

Secondary Outcome Measures

Name	Time	Method
Correlation between eosinophil content and body mass index (BMI)	baseline	Measured as a linear regression between BMI (defined as the weight in Kg/height in square m; kg/m2) and eosinophil number in the fat

Trial Locations

Locations (1)

Mayo Clinic; 🇺🇸 Scottsdale, Arizona, United States