The Subgingival Microbiota in Chronic Periodontitis Patients Affected by Type 2 Diabetes Mellitus: a Retrospective Comparative Study
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 40
- 试验地点
- 1
- 主要终点
- Bacterial load of six main periodontal pathogens
研究概览
简要总结
This study is a retrospective comparative evaluation of six main periodontal pathogens and total bacterial load in chronic periodontitis patients affected or not by type 2 diabetes mellitus by polymerase chain reaction analysis.
详细描述
The aim of this study was to compare prevalence and bacterial load of six main periodontal pathogens among chronic periodontitis patients with and without type 2 diabetes mellitus. Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans genotypes were also investigated.
The recruitment for the study sample was performed analysing the medical records of adult patients with chronic periodontitis visited between 2010 and 2016. Eligible patients were subdivided in two balanced groups on the basis of the presence or absence of type 2 diabetes mellitus at the time of the microbiological test.
For each subject, the following data have been collected: age, gender, smoking habits, type of metabolic control of diabetes, duration of diabetes, number of missing teeth.
For each subject, four periodontal sites were studied. Probing pocket depth, sites with bleeding on probing, sites with suppuration and microbiological data were evaluated.
An individual matching on the basis of severity and extension of periodontitis was performed by pairing a diabetic patient with another non-diabetic one with the same degree of severity and extension of the periodontal disease.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Retrospective
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •diagnosis of chronic periodontitis
- •diagnosis of type 2 diabetes mellitus (only in the test group)
- •the presence of at least 12 teeth (except for the third molar)
- •belonging to caucasian ethnic group
- •age greater than 18 years.
排除标准
- •pregnant or lactating
- •history of systemic antibiotics within 3 months prior to the microbiological testing or anti-inflammatory therapy in the month before the visit
- •if they were suffering from any other systemic disease except diabetes mellitus (arthritis, ulcerative colitis, Crohn's disease, osteoporosis or osteopenia, HIV infection, hematologic diseases, neoplastic diseases, cardiovascular diseases or pathologies that could potentially interfere with the periodontitis and/or with diabetes)
- •mental disorders
- •if they had received periodontal treatment in the 6 months before the microbiological test.
结局指标
主要结局
Bacterial load of six main periodontal pathogens
时间窗: November 16th 2016
Bacterial load mean of six main periodontal pathogens expressed as number after a polymerase chain reaction count
Prevalence of six main periodontal pathogens
时间窗: November 16th 2016
Number of sites presenting a specific pathogen
Total bacterial load
时间窗: November 16th 2016
A mean of all bacteria present in a periodontal site expressed as number after a polymerase chain reaction count
Relative bacterial load of six main periodontal pathogens
时间窗: November 16th 2016
A mean percentage that derives from the comparison of the bacterial load to the total bacterial load
次要结局
- Genotypes of Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans(November 16th 2016)
研究者
Marco Montevecchi
Aggregate Professor of Periodontology
University of Bologna