Effect of Ca2+ Ionophore on Embryonic Development and Clinical Outcome in Cases With Previous Fertilization Arrest

Not Applicable

• Conditions: Fertilization Arrest

• Registration Number: NCT02683031
• Lead Sponsor: TopLab Company for ART Laboratories Consultation and Training

Brief Summary

We aimed to investigate the efficiency of chemical oocyte activation using readymade Ca2+ ionophore on cases with previously cancelled cases due to fertilization arrest at pronuclear stage i.e: cleavage failure to find out if there is any improvement in embryonic developmental stages.

Detailed Description

Many studies reported that Ca2+ ionophore has a positive effect on embryo development in cases with complete or partial fertilization failure. However, only one preliminary report suggested a beneficial effect of Ca2+ ionophore activation in four cases with fertilization arrest.

Study Timeline

• Study Start: 2014-05
• Study First Submitted: 2016-02-11
• Study First Submitted QC: 2016-02-11
• Study First Posted: 2016-02-17
• Status Verified: 2016-08
• Last Update Submitted: 2016-08-17
• Last Update Posted: 2016-08-18
• Primary Completion: 2017-12
• Study Completion: 2017-12

Recruitment & Eligibility

• Status: UNKNOWN
• Sex: Female
• Target Recruitment: 50

Inclusion Criteria

• Patients with previous history of complete fertilization arrest.
• ovarian stimulation with the GnRH agonist and recombinant FSH in a long protocol.

Exclusion Criteria

• Female age >40 years

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Primary Outcome Measures

Name	Time	Method
Blastocyst formation rate	5 to 7days post oocyte retrival	Defined as the total number of blastocyst / total number of cleaved Embryos

Trial Locations

Locations (1)

Ahmed el-Damen; 🇪🇬 Cairo, Egypt