Co-culturing of primary chondrocytes and mesenchymal stem cells to optimize cartilage formatio

Completed

• Conditions: Cartilage damage; cartilage degeneration; 10043237

• Registration Number: NL-OMON31506
• Lead Sponsor: niversitair Medisch Centrum Utrecht

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Last Update Posted: 13 May 2024

Recruitment & Eligibility

• Status: Completed
• Sex: Not specified
• Target Recruitment: 48

Inclusion Criteria

-Male and female patients over 18 years of age
-Patients with a ruptured ACL admitted to ACL reconstructive surgery

Exclusion Criteria

-Patients over 40 years of age
-Patients with generalized cartilage degeneration

Study & Design

• Study Type: Observational invasive
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>Primary endpoint of this study will be after 6 weeks of in vitro culture or in<br /><br>vivo survival of the co-cultured chondrocytes and mesenchymal stem cells. At<br /><br>this time point we will analyze the ideal ratio of these cells for cartilage<br /><br>formation based on alcian blue staining, collagen propeptide I and II<br /><br>determination, safranin O staining and aggrecan ELISA.</p><br>

Secondary Outcome Measures

Name	Time	Method
<p>-</p><br>