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Subcutaneous Progesterone Versus Vaginal Progesterone for Endometrial Preparation in Fresh Donated Oocytes Recipients

Phase 4
Completed
Conditions
Infertility
Interventions
Registration Number
NCT02363127
Lead Sponsor
Instituto Bernabeu
Brief Summary

Randomised, prospective, investigator-blinded, controlled, single-centre study to assess the impact on the ongoing pregnancy rate with the use of two progesterones with different administration routes, in recipients of fresh embryos from donor oocytes, undergoing endometrial preparation for fresh embryo transfer.

Detailed Description

Exploratory study with a control group treated according to our Service's usual therapeutic regimen for the transfer of embryos with donor oocytes.

The controlled ovarian stimulation protocol in oocyte donors is always calculated according to the standard protocol at the Bernabeu Institute. Endometrial preparation will be carried out following the standard protocol of the Bernabeu Institute as follows: the oestrogen will be administered transdermally and patients with maintained ovarian function undergo medical hypophysectomy with depot GnRH agonists administered in the mid-luteal phase of the previous cycle.

On the day of oocyte retrieval, the patient will be randomised: Group A will be administered subcutaneous progesterone 25 mg/day (Prolutex), and Group B will be administered vaginal progesterone in capsules 200 mg/3 times a day (Progeffik).

The embryo transfer will be performed on day 5 of the embryo culture (Day +5). A biochemical pregnancy test beta- hCG and the P4 analysis will be performed 14 days after oocyte retrieval.

All the cycles will be monitored according to the Department's standard criteria, using transvaginal ultrasound to assess embryonic development and endometrial thickness, as well as analytical controls.

The study will be blinded to the investigator. The evaluating professionals will not know if the subject has been administered vaginal progesterone or subcutaneous progesterone. The medication will be delivered by a person who does not participate in the evaluations and who is dedicated to group assignment, to data centralisation, and to delivering the medication.

The aim of this study is to determine if the ongoing pregnancy rate in patients undergoing a fresh embryo transfer cycle with donor oocytes is affected by the progesterone administration route.

Recruitment & Eligibility

Status
COMPLETED
Sex
Female
Target Recruitment
120
Inclusion Criteria
  • Female, aged from 18 to 49 years (both inclusive)
  • Woman who wishes to become pregnant
  • Endometrial thickness greater 7 mm on the day of patient randomisation to one of the progesterone groups
  • Six or more donor retrieved oocytes
  • Patient programmed for fresh embryo transfer on day +5 of embryo culture
  • BMI lower than 30 Kg/m2
  • Infertility that justifies treatment with donor oocytes
  • Male with no known karyotype alterations
  • Semen by ejaculation from either the partner or from a bank
  • Uterus able to support embryo implantation and pregnancy
  • Absence of pregnancy before starting the embryo transfer cycle
  • Has given prior written consent
Exclusion Criteria
    • Important systemic diseases, endocrine-metabolic abnormalities involving the pituitary, thyroid, adrenals, pancreas, liver or kidney.
  • HIV, HBV or HCV seropositivity
  • Undiagnosed vaginal bleeding
  • Pregnancy, breastfeeding or any contraindication to becoming pregnant
  • Malformation of sexual organs incompatible with pregnancy
  • Known allergy to progesterone preparations or their excipients
  • Current dependence on alcohol, drugs or psychotropic medication
  • Concurrent participation in another study
  • Concomitant medication that could interfere with the study medication: different hormonal treatments used in the study, except thyroid hormones, antipsychotics, anxiolytics, hypnotics, sedatives, chronic treatment with prostaglandin inhibitors

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Prolutexsubcutaneous progesteroneSubcutaneous progesterone
Progeffikvaginal progesteroneVaginal progesterone
Primary Outcome Measures
NameTimeMethod
ongoing pregnancy rate at 12 weeks gestation12 weeks

ongoing pregnancy rate at 12 weeks gestation using subcutaneous progesterone and using vaginal progesterone capsules

Secondary Outcome Measures
NameTimeMethod
progesterone level on the days of the transfer5 days

progesterone level on the days of the transfer

progesterone level on biochemical pregnancy test beta-hCG14 days

progesterone level on biochemical pregnancy test beta-hCG

endometrium thickness on the day of oocyte retrieval0 day

endometrium thickness on the day of oocyte retrieval

endometrium thickness on the day of embryo transfer5 days

endometrium thickness on the day of embryo transfer

endometrium morphology on the day of oocyte retrieval0 day

endometrium morphology (trilaminar pattern and echogenicity) on the day of oocyte retrieval

endometrium morphology on the day of embryo transfer5 days

endometrium morphology (trilaminar pattern and echogenicity) on the day of embryo transfer

implantation rate4-5 weeks

number of embryo sacs at 4-5 weeks (by ultrasound) versus number of embryos transferred

positive biochemical pregnancy test beta- hCG rate14 days

positive biochemical pregnancy test beta- hCG rate at 14 days

clinical pregnancy rate4-5 weeks

rate of patients with embryo any sac with a heartbeat (by ultrasound)

miscarriage rate10 weeks

miscarriage rate in patients with positive biochemical pregnancy test beta- hCG rate on day +14

occurrence of side effectsday 5, day 14, 4-5 days, 10 weeks

occurrence of side effects associated with progesterone

comfort in relation to the progesterone administration route10 weeks

comfort in relation to the progesterone administration route

Trial Locations

Locations (1)

Instituto Bernabeu

🇪🇸

Alicante, Spain

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