Muscle Protein Metabolism in Obesity

Phase 1

Completed

• Conditions: Obesity
• Interventions: Other: Increase in the levels of plasma amino acids; Other: Aerobic exercise

• Registration Number: NCT01824173
• Lead Sponsor: Mayo Clinic

Brief Summary

Obesity is associated with reduced adenosine triphosphate (ATP) turnover in skeletal muscle, a condition that can impair muscle metabolism. The proposed research will discover mechanisms responsible for decreased content in mitochondrial proteins as well as in protein β-F1-ATPase, which is directly responsible for ATP assembly, in the muscle of obese individuals. This research will further examine the effectiveness of interventions, such as increased plasma amino acid availability and exercise, to increase the rate of production of mitochondrial proteins as well as that of β-F1-ATPase in the muscle of obese individuals. The findings will help to develop appropriate interventions to improve muscle ATP turnover and metabolism in obese people.

Detailed Description

Not available

Study Timeline

• Study Start: 2012-10
• Study First Submitted: 2013-04-01
• Study First Submitted QC: 2013-04-01
• Study First Posted: 2013-04-04
• Primary Completion: 2017-02-09
• Study Completion: 2020-02-07
• Status Verified: 2020-04
• Last Update Submitted: 2020-04-01
• Last Update Posted: 2020-04-03

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 84

Inclusion Criteria

Not provided

Exclusion Criteria

Not provided

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Amino Acid Infusion in Lean	Increase in the levels of plasma amino acids	Amino Acid Infusion in Lean
Exercise in Obese	Aerobic exercise	Exercise in Obese
Exercise in lean	Aerobic exercise	Exercise in lean
Amino acid Infusion in Obese	Increase in the levels of plasma amino acids	Amino acid Infusion in Obese

Primary Outcome Measures

Name	Time	Method
Rate of synthesis of muscle proteins (mixed muscle proteins, mitochondrial proteins, β-F1-ATPase)	Measured during a 9-hour infusion study

Secondary Outcome Measures

Name	Time	Method
β-F1-ATPase mRNA expression; PGC-1 expression	Measured during a 9-hour infusion study

Trial Locations

Locations (1)

Mayo Clinic in Arizona; 🇺🇸 Scottsdale, Arizona, United States