Endocrine Regulation of Lipolysis in Obesity and Diabetes

Not Applicable

Completed

• Conditions: Obesity; Diabetes
• Interventions: Other: adipose tissue sampling

• Registration Number: NCT02598544
• Lead Sponsor: Hasselt University

Brief Summary

Adipose tissue (AT) dysfunction is a commonly observed feature of metabolic dysfunction in obesity and diabetes. An important characteristic when examining AT function is the capacity to break down stored lipids (i.e. lipolysis) and its regulation. In the present study, the aim was to investigate whether atrial natriuretic peptide-mediated lipolysis is altered in different adipose tissue depots (subcutaneous and visceral adipose tissue) of obese subjects with or without type 2 diabetes, compared to age-matched lean men. Eventually, the knowledge gained from this research will contribute to a better understanding of the present adipose tissue dysfunction and to the optimization of exercise programs for people with obesity and diabetes.

Detailed Description

Not available

Study Timeline

• Study Start: 2013-05
• Primary Completion: 2015-04
• Study Completion: 2015-04
• Study First Submitted: 2015-10-16
• Study First Submitted QC: 2015-11-04
• Study First Posted: 2015-11-06
• Status Verified: 2016-11
• Last Update Submitted: 2016-11-16
• Last Update Posted: 2016-11-18

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 55

Inclusion Criteria

• Lean group: BMI < 25 kg/m², insulin sensitive (HOMA-Insuline Resistance (IR) < 2.3), age 40-65
• Obese groups: BMI > 30 kg/m², presence or absence of type 2 diabetes based on use of glucose lowering medication or HbA1c > 6.5%, age 40-65

Exclusion Criteria

• presence of a history of heart, lung or kidney disease and/or presence of endocrine anomalies. Use of beta-blockade medication.

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Obese men with type 2 diabetes	adipose tissue sampling	-
Obese men without type 2 diabetes	adipose tissue sampling	-
lean men	adipose tissue sampling	-

Primary Outcome Measures

Name	Time	Method
Glycerol release by mature adipocytes, isolated from subcutaneous and visceral adipose tissue, upon stimulation with isoprenalin or ANP (ex vivo)	day 1	Investigation of concentration effect of ISO/ANP on lipolysis (glycerol release) by mature adipocytes isolated from adipose tissue biopsies, glycerol release is measured in the incubation medium following a 3 hour incubation period. Measured for adipose tissue (subcutaneous and visceral adipose tissue) of both lean and obese subjects
ANP receptor expression profiles on mRNA and protein level in both adipose tissue depots for both lean and obese subjects.	day 1	Receptor expression determination in order to clarify the underlying mechanism responsible for possible differences in lipolytic responses.
Adipose tissue inflammation	day 1	Adipose tissue inflammation (by means of FACS analysis) and its relation to insulin sensitivity and adipose tissue function in lean, obese and obese diabetics subjects.

Secondary Outcome Measures

Name	Time	Method
blood triglycerides	day 1
blood pressure (mmHg)	Day 1
waist (cm)	Day 1
blood glucose	day 1
blood insuline	day 1
blood FFA	day 1
blood glycated hemoglobin	day 1
Concomitant medication	Day 1
Body composition	day 1	Body composition (body fath percentage), measured by BIA
BMI (kg/m²)	day 1
body height (m)	day 1
weight (kg)	day 1
hip circumference (cm)	Day 1
blood ANP	day 1	fasting blood sample to determine blood glucose, insulin, FFA, , glycated hemoglobin and ANP

Trial Locations

Locations (4)

Ziekenhuis Oost-Limburg; 🇧🇪 Genk, Belgium

Jessa Ziekenhuis; 🇧🇪 Hasselt, Belgium

Maastricht University; 🇳🇱 Maastricht, Netherlands

Hasselt University; 🇧🇪 Diepenbeek, Belgium