Comparison of the Methylation Pattern of DNA in Buccal Swabs From Different Time Points

Terminated

• Conditions: DNA Methylation
• Interventions: Procedure: Buccal swab

• Registration Number: NCT02297009
• Lead Sponsor: Universitaire Ziekenhuizen KU Leuven

Brief Summary

A buccal swab will be taken using a cotton stick by rubbing it against the buccal mucosa. Samples will be taken from the same participant at different timepoints.

DNA will be extracted from the buccal cells on the swabs using a commercial extraction kit and will be quantified by a nano-drop spectrophotometer.

We will determine global and gene-specific methylation and hydroxymethylation alterations by UPLC/MS/MS. Hydrolyzed DNA into individual nucleosides will be analyzed for the quantitative measurement of 5-methylCytosine and 5-hydroxymethylCytosine on Triple Quadrupole UPLC/MS/MS platform. Secondly, specific methylation in the CpG islands of tumor suppressor and promoter genes, and genes involved in the oxidative stress pathways will be assessed by PCR-pyrosequencing.

Detailed Description

Not available

Study Timeline

• Study First Submitted: 2014-10-31
• Study First Submitted QC: 2014-11-18
• Study First Posted: 2014-11-21
• Study Start: 2014-12
• Status Verified: 2024-07
• Primary Completion: 2024-07
• Study Completion: 2024-07
• Last Update Submitted: 2024-07-12
• Last Update Posted: 2024-07-16

Recruitment & Eligibility

• Status: TERMINATED
• Sex: All
• Target Recruitment: 20

Inclusion Criteria

• Caucasian

Exclusion Criteria

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
Volunteers	Buccal swab	30 volunteers, half men and half women Buccal swab

Primary Outcome Measures

Name	Time	Method
%Methylation (%)	5 weeks

Secondary Outcome Measures

Name	Time	Method
%Hydroxymethylation (%)	5 weeks

Trial Locations

Locations (1)

BIOMAT; 🇧🇪 Leuven, Belgium