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CNS10-NPC-GDNF Delivered to the Motor Cortex for ALS

Phase 1
Recruiting
Conditions
Amyotrophic Lateral Sclerosis
Interventions
Biological: CNS10-NPC-GDNF
Registration Number
NCT05306457
Lead Sponsor
Cedars-Sinai Medical Center
Brief Summary

The investigator is examining the safety of transplanting cells, that have been engineered to produce a growth factor, into the motor cortex (brain) of patients with Amyotrophic Lateral Sclerosis (ALS). The cells are called neural progenitor cells, which are a type of stem cell that can become several different types of cells in the nervous system. These cells have been derived to specifically become astrocytes, which is a type of neural cell. The growth factor is called glial cell line-derived neurotrophic factor, or GDNF. GDNF is a protein that promotes the survival of many types of neural cells. Therefore, the cells are called "CNS10-NPC-GDNF." The investigational treatment has been tested in people by delivering it to the spinal cord. However, it has only been delivered to the motor cortex of animals. In this study, we want to learn if CNS10-NPC-GDNF cells are safe to transplant into the motor cortex (brain) of people.

Detailed Description

This study will be the first to use a genetically modified progenitor cell line delivered to the motor cortex to treat a neurodegenerative disease. This is a Phase 1/2a, single-center, safety study of two escalating doses of human neural progenitor cells expressing GDNF (CNS10-NPC-GDNF) delivered unilaterally to the "hand-knob" area of the motor cortex of patients with ALS.

Subjects meeting all Eligibility Criteria and providing Informed Consent will be enrolled in one of three sequential dosing groups. Subjects will be treated sequentially with a minimum of one month interval between surgeries for the first three subjects in each dosing cohort. The remaining subjects in the cohort will be treated with a minimum interval of at least one week between surgeries.

Primary Outcome:

Safety, as evaluated by:

* Adverse Events and Serious Adverse Events

* Post-op MRI and/or CT (with contrast) and as clinically indicated

Recruitment & Eligibility

Status
RECRUITING
Sex
All
Target Recruitment
16
Inclusion Criteria

Not provided

Exclusion Criteria

Not provided

Study & Design

Study Type
INTERVENTIONAL
Study Design
SEQUENTIAL
Arm && Interventions
GroupInterventionDescription
CNS10-NPC-GDNF - Group CCNS10-NPC-GDNFUnilateral Motor Cortex, 0.5x10\^6 cells in 10 µL/site, 21 sites (10.5x10\^6 total cells) - Motor cortex corresponding to the dominant hand
CNS10-NPC-GDNF - Group ACNS10-NPC-GDNFUnilateral, Motor Cortex, 0.25x10\^6 cells in 10 µL/site, 21 sites (5.25x10\^6 total cells) - Motor cortex corresponding to the non-dominant hand
CNS10-NPC-GDNF - Group BCNS10-NPC-GDNFUnilateral, Motor Cortex, 0.5x10\^6 cells in 10 µL/site, 21 sites (10.5x10\^6 total cells) - Motor cortex corresponding to the non-dominant hand
Primary Outcome Measures
NameTimeMethod
Safety, as evaluated by the incidence of Adverse Events and Serious Adverse Events and their relationship to the treatment12 months post-operatively
Safety, as evaluated by changes from baseline in the brain MRI12 months post-operatively
Secondary Outcome Measures
NameTimeMethod
Pinch StrengthPinch Strength testing will be performed 7 times over 15 months

Change from baseline for pinch strength

Force Generation via Accurate Test of Limb Isometric Strength (ATLIS) testingATLIS testing will be performed 7 times over 15 months

Change from baseline for force generation by ATLIS

Functional Hand assessments using 9-hole peg test9-hole peg testing will be performed 7 times over 15 months

Change from baseline for 9-hole peg test

Penn Upper Motor Neuron Score (PUMNS)PUMNS will be performed 7 times over 15 months

Change from baseline for Penn Upper Motor Neuron Score. (Scale of 0-32, where 0 is normal)

Hand/Wrist StrengthHand/Wrist strength testing will be performed 7 times over 15 months

Change from baseline for Hand/Wrist strength using Hand-held dynamometer

Compound Motor Action Potential (CMAP)CMAP will be performed 7 times over 15 months

Change from baseline for CMAP

Hand Knob - Functional MRI (fMRI)fMRI will be performed up to 4 times over 15 months

Changes from baseline in brain activity in the hand knob area evaluated by fMRI

Trial Locations

Locations (1)

Cedars-Sinai Medical Center

🇺🇸

Los Angeles, California, United States

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