The Effect of Exercise on Myokine Production in Aging Persons

Not Applicable

Recruiting

• Conditions: Exercise; Aging

• Registration Number: NCT05571709
• Lead Sponsor: Hasselt University

Brief Summary

The incidence of age-related diseases, such as cancer and cardiovascular diseases, rapidly rises. These days, age-related diseases cause the majority of all healthcare expenses. During ageing senescent cells secrete a range of harmful inflammatory signals, which leads to chronic inflammation (inflamm-aging) and accelerates aging throughout the rest of the body. Interestingly, people who regularly exercise show less signs of inflamm-aging compared to people with a sedentary lifestyle. Molecules secreted by skeletal muscles, called myokines, may play an important role. However, the underlying molecular mechanisms remain unknown. This pilot study aims to investigate if an acute bout of resistance training is an appropriate tool to induce an increase in the release of muscle derived myokines in aged persons.

Detailed Description

Not available

Study Timeline

• Study Start: 2022-09-15
• Study First Submitted: 2022-10-05
• Study First Submitted QC: 2022-10-06
• Study First Posted: 2022-10-07
• Status Verified: 2023-03
• Last Update Submitted: 2023-03-29
• Last Update Posted: 2023-03-31
• Primary Completion: 2023-10-15
• Study Completion: 2023-12-31

Recruitment & Eligibility

• Status: RECRUITING
• Sex: All
• Target Recruitment: 20

Inclusion Criteria

• Healthy persons

Exclusion Criteria

• Smoking

• Infection < 2 weeks ago

• Received a vaccine < 2 weeks ago

• Allergy to Lidocain

• Immune deficiency

• Presence of a tumour or in treatment

• Usage of anti-coagulant medication

• Presence of following conditions:

  • Haemophilia
  • Auto-immune disease
  • Cardiovascular disease
  • Musculoskeletal diseases which refrains the patient from exercising

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Primary Outcome Measures

Name	Time	Method
Cytokine concentration	Day 2	Measurement of multiple cytokines in blood (for example: IL-6, IL-15, IL-4, irisin...) with ELISA's or multiplex assays
Myokine production	Day 2	Immunohistochemistry staining (fluorescent) of multiple myokines (for example: IL-6, IL-15, IL-4, irisin..) and aging markers (p16,p21...) on muscle tissue
Myokine protein levels	Day 2	Western blotting of multiple myokines in muscle tissue
Myokine gene expression	Day 2	Quantitative PCR (polymerase chain reaction) of markers in muscle tissue
Immune parameters	Day 2	Flow cytometry panels of immune parameters in muscle tissue

Secondary Outcome Measures

Name	Time	Method
Resting Heart Rate	Day 1	Measured using an electronic sphygmomanometer
Blood pressure	Day 1	The force of circulating blood on the walls of the arteries. Measured using an electronic sphygmomanometer
International Physical Activity Questionnaire (Short form)	Day 1	Measures the subjective level of activity of the last seven days. 7 questions regarding amount of time spent on certain activities.

Trial Locations

Locations (1)

Hasselt University; 🇧🇪 Diepenbeek, Limburg, Belgium