Effects of Microbouts of Activity on Metabolic Health
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 30
- 试验地点
- 2
- 主要终点
- Changes in sedentary time as measured by ActiPAL
研究概览
简要总结
Population studies suggest that time spent in sedentary behaviors is associated with all-cause mortality including obesity, diabetes, and cardiovascular diseases, independent of time spent in exercise. Frequent interruptions to sedentary time are however beneficially associated with metabolic health outcomes, even in individuals who exercise regularly. The goal is to use integrative approach to understand the biological mechanisms that underlie these associations in a longitudinal intervention study in overweight sedentary adults. The investigators believe that the proposed study will provide an initial evidence base for the health benefits of breaking up prolonged sitting with short bursts of activity. This innovative strategy may be more effective at combating the adverse effects of sedentary behaviors than more traditional approaches.
详细描述
To compare the effects of 6-weeks of microbursts of moderate-intensity activity to a calorically equivalent single bout of 45 minutes of moderate-intensity exercise training, both performed 5 days per week for a total of 45 minutes, in free-living sedentary overweight adults on:
Aim 1: plasma inflammatory and cardiometabolic health biomarkers in association with total energy expenditure and time spent in sedentary behaviors.
H1: Microbursts of activity will result in higher daily energy expenditure and lower time spent sedentary, as respectively measured with doubly labeled water and accelerometry, than traditional exercise training. This will be associated with healthier inflammatory Interleukin 1 alpha (IL1a), Interleukin 6 (IL6), Interleukin 10 (IL10), Tumour Necrosis Factor alpha (TNFa) and cardiometabolic (triglycerides, cholesterol, C-Reactive Protein (CRP), High-density lipoprotein (HDL), and Low-density lipoprotein (LDL) profiles compared to those obtained with traditional exercise training program.
Aim 2: 24-hr total fat and carbohydrate oxidation, exogenous carbohydrate oxidation, dietary fat trafficking between oxidation and incorporation into muscle lipid fractions, and mitochondrial function.
H2: Both one single continuous bout and microbouts of activity will increase 24-hr total (whole-room calorimetry) and dietary (D31-palmitate) fat oxidation due to greater mitochondrial lipid oxidation (high resolution respirometry) and lower incorporation of dietary fatty acid into muscle lipid fractions (D31-palmitate), as compared to baseline sedentary control condition. Microbouts of activity will further result in greater increases in 24-hr total (whole-room calorimetry) and exogenous (U-13C6 glucose) carbohydrate oxidation and in mitochondrial carbohydrate-linked oxidation, than traditional isocaloric exercise training.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 45 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Overweight but weight stable (+/- 3kg over previous 6 months) sedentary male and female adults (n=30) will be recruited.
- •Ages between 18-45 years old
- •BMI of 25-35 kg/m2
- •Sedentary ( self report < 150min/wk of moderate-to-vigorous activity or less than 6,500 steps per day as measured for 5 days in free-living conditions with a pedometer).
- •The use of birth pill control will be accepted
- •Exclusion criteria:
- •Any history of renal (present or past kidney failure, kidney transplant, dialysis or kidney cysts),
- •Uncontrolled hypertension
- •Cardiovascular (present or past atherosclerosis, heart attack, ischemic stroke, heart failure)
- •Hepatic diseases (past or present hepatitis B or C, fibrosis, cirrhosis, NAFLD/NASH)
- •Type 1 or 2 diabetes
- •Consumption of drugs (marijuana included)
- •Consumption of alcohol (>40g/d)
- •HIV positivity
- •Psychiatric disorders
- •Any medications known to interfere with lipid or energy metabolism
- •Known physical activity contraindications
- •Major illness/physical problems (acute or chronic) that may limit their ability to perform the walking activities
排除标准
- 未提供
结局指标
主要结局
Changes in sedentary time as measured by ActiPAL
时间窗: weeks 1-2 and weeks 5-6
Over each 2 week period, participants will be instructed to wear an ActiPAL (PALTechnologies: Glasgow, Scotland) activity monitor on their right leg. Participants will be instructed to wear the monitors at all times except when sleeping or participating in water-based activities. The time-stamped "event" data file from the activPAL software will be used to determine time spent sitting/lying, standing and stepping per day.
Changes in insulin sensitivity
时间窗: Week 2 and week 8
Insulin sensitivity will be determined via an insulin modified frequently sampled intra-venous glucose tolerance test (IVGTT). After baseline samples, intravenous glucose (0.3 g/kg) will be infused over 1 minute, followed by insulin at 0.03 U/kg, 20 minutes after glucose administration. Blood samples will be frequently sampled over 5 hours, and insulin sensitivity will be calculated using the Bergman minimal model.
Postprandial glycemia
时间窗: Week 2 and week 8
Incremental area under the curve of plasma glucose concentration measured in fasting and every hour for 14 hours.
次要结局
- Changes in total energy expenditure (TEE)(week 1-2 and week 5-6)
- Changes in Moderate-to-vigorous physical activity as measured by ActiGraph(weeks 1-6)
- 24hr substrate oxidation and energy expenditure(Week 2 and week 8)
- Changes in Dietary Carbohydrate Oxidation(week 2 and week 8)
- Changes in Dietary Fat Oxidation(week 2 and week 8)
- Postprandial insulinemia(Week 2 and week 8)
- Postprandial NEFA(Week 2 and week 8)
- Postprandial triglyceridemia(Week 2 and week 8)
- Daily glucose mean and oscillation(Weeks 1-2 and weeks 5-6)
- Changes in Mitochondria function(week 2 and week 7)
- Changes in plasma inflammatory markers(Week 2 and week 8)
- changes in plasma total cholesterol, HDL and LDL(Week 2 and week 8)