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Effects of Pulse Consumption on Bone Health of Postmenopausal Women

Not Applicable
Completed
Conditions
Menopause
Interventions
Other: pulses
Registration Number
NCT04692298
Lead Sponsor
Oklahoma State University
Brief Summary

This study will investigate the effects of the addition of 100 grams/day of cooked pulses (i.e. lentil, pinto beans, peas, chickpeas, kidney beans) to the diet of postmenopausal women for 12 weeks on gut health, metabolic outcomes and bone biomarkers.

Detailed Description

With approximately 1.3 million women reaching menopause each year in the US and about one-third of a woman's life is spent in this state, it is imperative to identify effective, safe, and economical approaches that can minimize disease risk that is associated with this phase of life. Pulses are excellent source of fiber, protein, essential amino acids, vitamins, minerals and phytochemicals, that can act as prebiotics and prevent gut dysbiosis and promote a healthy gut. A few studies in overweight or obese adults have shown the health benefits of pulses, including gut modulating potential. However, studies examining the use of pulse crops are limited, especially in alleviating health risks associated with menopause. The objective of this study is to evaluate the prebiotic potential of pulse-based diet and consequent effects on metabolic and bone biomarkers in postmenopausal women. We hypothesize that daily intake of pulses, due to its nutrient content and many other bioactive compounds including fiber content, will beneficially affect gut health and subsequently improve metabolic outcomes and bone markers in postmenopausal women. To accomplish our objectives, 40 postmenopausal women (50- 65 y old and ≥ 1 y menopause) will be recruited and will be asked to consume 100 g/d of pulse (alternate between lentils, pinto beans, peas, chickpeas, and kidney beans) for 3 months. Pulse intake, anthropometric measures, markers of gut and bone health, and metabolic outcomes will be assessed at baseline and at the end of pulse consumption.

Once the study was underway and we got more funding, women were given the option to continue pulse supplementation for another six months to examine changes on bone density.

Recruitment & Eligibility

Status
COMPLETED
Sex
Female
Target Recruitment
41
Inclusion Criteria
  • 1 year without menstrual cycle
Read More
Exclusion Criteria
  • any medication use (for the past 6 m) that affects glucose, lipids, bone and inflammation markers, dietary supplements, and non-steroidal anti-inflammatory medications
  • allergy to pulse crops
  • tobacco use
  • excessive alcohol intake
  • antibiotic use
  • major surgery within 6 m of study enrollment
Read More

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
PulsepulsesParticipants will consume kidney beans, lentil, pinto beans, black-eyed pea, chickpea
Primary Outcome Measures
NameTimeMethod
blood C-terminal telopeptide of type 1 collagen (CTX)change from baseline after 90 days

analyzed by enzyme-linked immunoassay

fecal short chain fatty acidchange from baseline after 90 days

analyzed by gas chromatography

blood procollagen type 1 N-propeptide (P1NP)change from baseline after 90 days

analyzed by enzyme-linked immunoassay

fecal immunoglobulin Achange from baseline after 90 days

analyzed by enzyme-linked immunoassay

changes in blood biomarkers and/or bone mineral density (BMD)change from baseline, after 90 days and 9 months (for BMD)

analyzed by enzyme linked immunoassay or dual energy xray absorptiometry for BMD

fecal bacteriachange from baseline after 90 days

analyzed by 16sRNA sequencing

plasma concentrations of fatty acid binding proteinchange from baseline after 90 days

analyzed by enzyme-linked immunoassay

Secondary Outcome Measures
NameTimeMethod
blood triglycerideschange from baseline after 90 days

analyzed using clinical chemistry analyzer

fasting blood glucosechange from baseline after 90 days

analyzed using clinical chemistry analyzer

blood insulinchange from baseline after 90 days

analyzed using enzyme linked immunoassay

blood C-peptidechange from baseline after 90 days

analyzed using enzyme linked immunoassay

blood glycosylated hemoglobinchange from baseline after 90 days

analyzed using clinical chemistry analyzer

blood total cholesterolchange from baseline after 90 days

analyzed using clinical chemistry analyzer

blood LDL cholesterolchange from baseline after 90 days

analyzed using clinical chemistry analyzer

blood HDL cholesterolchange from baseline after 90 days

analyzed using clinical chemistry analyzer

Trial Locations

Locations (1)

Nutritional Sciences Department, Oklahoma State University

🇺🇸

Stillwater, Oklahoma, United States

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