The Age-related Changes of Energy Metabolism in Physically Fit Adult Males

Not Applicable

Completed

• Conditions: Healthy
• Interventions: Diagnostic Test: Cardio-pulmonary exercise test

• Registration Number: NCT05113914
• Lead Sponsor: Poznan University of Physical Education

Brief Summary

The influence of age and physical fitness level on the level of energy metabolism and antioxidant capacity in adult males.

Detailed Description

Over four hundred healthy, adult males of different age categories and physical fitness levels were included in the study. There is much research convincing that in athletes of various sports disciplines the level of energy metabolism, oxidative stress, and amino acid profiles during years of training undergoes sports adaptation. The study aimed to evaluate how age and physical fitness level affects the level of purine metabolites and metabolites of nicotinamide adenine dinucleotide, the level of antioxidant capacity, and amino acid profiles. Investigators hypothesized that there will be an age-related decline in most of the physiological indices, but significant differences will appear regarding the level of an individual's sports adaptation.

Study Timeline

• Study Start: 2011-01-01
• Primary Completion: 2019-11-30
• Study Completion: 2019-12-20
• Status Verified: 2021-09
• Study First Submitted: 2021-10-07
• Study First Submitted QC: 2021-10-29
• Study First Posted: 2021-11-09
• Last Update Submitted: 2021-11-10
• Last Update Posted: 2021-11-16

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 420

Inclusion Criteria

1. healthy
2. non-smoking
3. physically fit, including top-level athletes

Exclusion Criteria

1. no reported history of a cardiopulmonary or renal diseases, and other chronic diseases
2. no major orthopedic injury or illness resulting in an inability to run
3. no medications that could affect cardiopulmonary functions
4. normal resting electrocardiogram
5. body mass index (BMI) below 30.0 kg·m-2
6. normal erythrocyte count and Hb content
7. no pathological states known of significantly elevated adenylate pool and concentration of ATP, e.g. sickle cell disease, diabetes, leukemia, sepsis, tuberculosis, meningococcal infection, or renal insufficiency

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
Healthy, physically fit men	Cardio-pulmonary exercise test	All participants underwent a single CPET test with pre- and post-exercise blood measurements.

Primary Outcome Measures

Name	Time	Method
Cardio-respiratory measures	during the intervention	Cardiopulmonary exercise test (CPET) was conducted while an incremental running test on the treadmill using a portable breath-by-breath ergospirometer and heart rate monitor.

Secondary Outcome Measures

Name	Time	Method
Glutathione peroxidase (GPx) activity	baseline	The measurement of glutathione peroxidase (GPx) activity was performed using commercially available ELISA immunoassay.
Blood Lactate level	baseline and immediately after the intervention	Lactate concentration was performed by the spectrophotometric enzymatic method.
Total antioxidant status (TAS)	baseline	Total antioxidant status (TAS) measurement was performed using commercially available ELISA immunoassay.
Plasma purine nucleotides	baseline and immediately after the intervention	The plasma purine metabolites were estimated in plasma using high-performance liquid chromatography (HPLC) with UV-VIS detection.; Measurements performed: Guanosine, Inosine, Adenosine, Hypoxanthine, Xanthine, Uric Acid, Allantoin, Adenine Nucleotides \[adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), inosine monophasphate (IMP)\], Guanine Nucleotides \[guanosine triphosphate (GTP), guanosine diphosphate (GDP), guanosine monophosphate (GMP)\].
Erythrocyte Purine Metabolites	baseline and immediately after the intervention	The erythrocyte purine metabolites were estimated in erythrocytes using high-performance liquid chromatography (HPLC) with UV-VIS detection.; Measurements performed: Adenine Nucleotides \[adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), inosine monophasphate (IMP)\], Guanine Nucleotides \[guanosine triphosphate (GTP), guanosine diphosphate (GDP), guanosine monophosphate (GMP)\].
Malondialdehyde (MDA)	baseline	The measurement of malondialdehyde (MDA) was performed using commercially available ELISA immunoassay.
Aminoacid profile	baseline	The levels of 20 aminoacids were estimated in plasma using mass spectrometric procedure.
Nicotinamide Adenine Dinucleotide (NAD) and its metabolites	baseline and immediately after the intervention	Nicotinamide Adenine Dinucleotide (NAD) and its metabolites were estimated in plasma using mass spectrometric procedure.; Measurements performed: Nicotinamide Adenine Dinucleotide (NAD+), Nicotinamide (NA), 1-Methylnicotinamide (MNA), 1-methyl-2-pyridone-5-carboxamide (M2PY), 1-methyl-4-pyridone-5-carboxamide (M4PY), Nicotinamide Mononucleotide (NMN), 4-pyridone-3-carboxamide ribonucleoside (4PYR)
Reduced glutathione (GSH)	baseline	The measurement of reduced glutathione (GSH) was performed using commercially available ELISA immunoassay.
Hypoxanthine-guanine phosphoribosyltransferase (HGPRT) activity	baseline and immediately after the intervention	The HGPRT activity was estimated in whole blood and in erythrocytes using high-performance liquid chromatography (HPLC) with UV-VIS detection.
Thiobarbituric-acid-reactive substances (TBARS)	baseline	The measurement of thiobarbituric-acid-reactive substances (TBARS) was performed using commercially available ELISA immunoassay.
Polyphenols	baseline	The measurement of polyphenols was performed using commercially available ELISA immunoassay.

Trial Locations

Locations (1)

Poznan University of Physical Education; 🇵🇱 Poznań, Poland