Impact of Exercise Combined With Inulin Propionate Ester on Fat Oxidation

Not Applicable

Completed

• Conditions: Exercise; Inulin Propionate Ester
• Interventions: Behavioral: Exercise training with Inulin Propionate Ester; Behavioral: Exercise training with placebo

• Registration Number: NCT04016350
• Lead Sponsor: University of Glasgow

Brief Summary

There is evidence to suggest that responsiveness to exercise training programmes can be expected to be influenced by changes in resting fat oxidation, an important factor in the aetiology of obesity. Our previous work has shown that oral supplementation with inulin propionate ester (IPE) reduces intra-abdominal fat and prevents weight gain and that oral propionate intake enhances resting fat oxidation. The effects of IPE combined with exercise training on resting fat oxidation and body fatness are unknown. The aim of this study was to investigate the impact of 4-weeks IPE supplementation, in combination with a moderate intensity exercise training programme, on whole body fat oxidation and on plasma GLP-1 and PYY.

Detailed Description

Participants: Healthy overweight females with BMI \>25 kg/m2 and 25-45 years of age were recruited through advertisements and word of mouth on the campus of the University of Glasgow or from other public places. Participants were required to be sedentary, non-smokers, with stable body weight for two months prior to the study enrollment, not pregnant, free of medication, nutritional supplementation or following any specific diet and with no antibiotic use for the past three months. Participants with chronic illness, eating disorders and history of gastrointestinal operations were excluded.

Study Design: This is a randomized study with a parallel design. Study participants underwent 4 week supervised moderate intensity exercise training combined either with IPE (EX/IPE) or cellulose as placebo (EX/Placebo) supplementation at doses of 10g / day. Supplements were provided as sachets containing white powder and participants were asked to consume one sachet per day with breakfast in a way convenient for each participant. Before the start of the study and at the end of the 4-week intervention, participants were asked to conduct a submaximal exercise test. Prior to the first and after the second submaximal exercise test, participants of both groups conducted a 7-hour experimental trial which involved collection of expired air and blood samples in fasted and postprandial states. Body weight and body composition were measured in the fasted state. Prior to the first 7-hour experimental trial, participants were asked to record their diet for 3 days and replicate this intake prior to the second 7-hour experimental trial.

Study Timeline

• Study Start: 2015-02-05
• Primary Completion: 2017-03-10
• Study Completion: 2017-06-11
• Status Verified: 2019-07
• Study First Submitted: 2019-07-05
• Study First Submitted QC: 2019-07-09
• Last Update Submitted: 2019-07-09
• Study First Posted: 2019-07-11
• Last Update Posted: 2019-07-11

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Female
• Target Recruitment: 20

Inclusion Criteria

Healthy, sedentary and overweight females with BMI >25 kg/m2 and stable body weight for two months prior to the study.

Exclusion Criteria

Acute or chronic illness, eating disorders, history of gastrointestinal operations, smoking, pregnancy, taking supplements, and being on specific diet for the past three months.

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Exercise Training with Inulin Propionate Ester	Exercise training with Inulin Propionate Ester	Study participants underwent 4 week supervised moderate intensity exercise training combined with Inulin Propionate Ester supplementation at doses of 10g / day.
Exercise Training with Placebo	Exercise training with placebo	Study participants underwent 4 week supervised moderate intensity exercise training combined with cellulose as placebo at doses of 10g / day.

Primary Outcome Measures

Name	Time	Method
Resting fat oxidation	4 weeks	Changes in resting fat oxidation measured in the fasted and postprandial states by means of indirect calorimetry (Oxycon Pro, Germany).
Metabolic rate	4 weeks	Changes in resting energy expenditure measured in the fasted and postprandial states by means of indirect calorimetry (Oxycon Pro, Germany).
Resting carbohydrate oxidation	4 weeks	Changes in resting fat oxidation measured in the fasted and postprandial states by means of indirect calorimetry (Oxycon Pro, Germany).

Secondary Outcome Measures

Name	Time	Method
Height	4 weeks	Height (meters)measured by stadiometer, a vertical ruler with a sliding horizontal paddle which is adjusted to rest on the top of the head.
Body weight	4 weeks	Changes in body weight measured by TANITA scales (TBF-300).
Glucagon-like peptide-1 (GLP-1)	4 weeks	Changes in plasma concentration of fasting and postprandial GLP-1 measured using ELISA (Merc Millipore) kit.
Body fat (%)	4 weeks	Changes in body fat expressed as percentage (proportion of fat to the total body weight) measured by TANITA scales (TBF-300).
Maximal oxygen consumption [Time Frame: 4 weeks]	4 weeks	Changes in maximal oxygen consumption (ml/kg/min) measured by Servomex Gas Analyser.
Peptide YY (PYY)	4 weeks	Changes in plasma concentration of fasting and postprandial PYY measured using ELISA (Merc Millipore) kit.