The Methylation Level of Interspersed Repetitive Sequences in Human Dental Pulp Stem Cell-derived Osteoblasts

Not Applicable

Recruiting

• Conditions: INE- 1, Alu and HERV- K methylation level and pattern; Epigenetic, DNA methylation, Intersperse repetitive sequence, Dental pulp stem cell, Osteoblast

• Registration Number: TCTR20181119001
• Lead Sponsor: Chulalongkorn University

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Study Start: 2018-11-19
• Study Completion: 2018-12-27
• Last Update Posted: 19 August 2024

Recruitment & Eligibility

• Status: Recruiting
• Sex: All
• Target Recruitment: 13

Inclusion Criteria

The non-pathological third molars removed from healthy adult subjects (N=13) who seek treatment for impacted third molars at the Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Chulalongkorn University will be used. After the consents for this study are signed, the dental pulp tissue will then be isolated in sterile condition

Exclusion Criteria

1.Patient whose third molars have pathology
2.Patient whose third molars are needed to odontectomy

Study & Design

• Study Type: Observational
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
INE-1, Alu and HERV-K methylation level 14 days after differentiated dental pulp stem cell to osteoblast quantitative combined bisulfite restriction analysis(qCOBRA) technique

Secondary Outcome Measures

Name	Time	Method
INE-1, Alu and HERV-K methylation pattern 14 days after differentiated dental pulp stem cell to osteoblast quantitative combined bisulfite restriction analysis(qCOBRA) technique