The Association Between Food Timing and Composition on Gastrointestinal Injury, Stress Hormone and Gastrointestinal Complains.
- Conditions
- Endothelial Dysfunction
- Registration Number
- NCT06403241
- Lead Sponsor
- Poznan University of Physical Education
- Brief Summary
The study aimed to check the influence of timing and composition of diet on markers of gut injury, endotoxemia and stress.
- Detailed Description
The research protocol includes detailed food intake analysis, including timing and composition, markers related to the neuroendocrine pathway cortisol, and observations of individually experienced symptoms. Moreover, the study also explores intestinal injury and endotoxemia parameters, such as I-FABP, CLDN-3, and LBP. As a first goal, this study investigates the timing and composition of food intake concerning changes in gut integrity, cortisol levels, and gastrointestinal symptoms. Investigators explored whether an athlete's nutritional intake influences any GI disturbance induced by high-intensity exercise. As a second goal, Investigators tried to connect symptoms and markers from both pathways with gastrointestinal complaints. Investigators hypothesised that cortisol levels would be more profound than other studied parameters in gastrointestinal symptoms.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- Male
- Target Recruitment
- 13
- Membership in Polish Rowing Team,
- minimum five years of training,
- total training time minimum of 240 minutes,
- filling out a food diary,
- finishing 2000-meter ergometer test.
- Probiotics within the last three months,
- prebiotics within the last three months
- antibiotic therapy within the last three months,
- dietary regime,
- gastrointestinal diseases,
- lactose intolerance
Study & Design
- Study Type
- OBSERVATIONAL
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method Corisol Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test, and 1-hour recovery period Stress hormoneconcentration measured in blood \[ng/ml\] using commercially available enzyme-linked immunosorbent assays
I-FABP (intestinal fatty acid binding protein)to measure epithelial wall injury Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test, and 1-hour recovery period I-FABP concentration measured in blood \[ng/ml\] using commercially available enzyme-linked immunosorbent assays
Claudin to measure tight junction leakage Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test, and 1-hour recovery period Zonulin concentration measured in blood \[ng/ml\] using commercially available enzyme-linked immunosorbent assays
LBP (lipopolysaccharide binding protein) to measure endotoxin Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test, and 1-hour recovery period LBP concentration measured in blood \[ug/ml\] using commercially available enzyme-linked immunosorbent assays
Lactic acid to measure fatigue after the race Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test Lactate concentration measured in capillary blood from earlobe \[mmol/L\] using a commercially available kit (Diaglobal, Berlin, Germany)
- Secondary Outcome Measures
Name Time Method protein whole day (24hours) before test, in the morning before the test protein \[g \]of food intake measured by food diary
fat whole day (24hours) before test, in the morning before the test fat \[g \]of food intake measured by food diary
glucose whole day (24hours) before test, in the morning before the test glucose \[g \]of food intake measured by food diary
energy whole day (24hours) before test, in the morning before the test energy \[kcal \]of food intake measured by food diary
carbohydrate whole day (24hours) before test, in the morning before the test carbohydrate \[g \]of food intake measured by food diary
fructose whole day (24hours) before test, in the morning before the test fructose \[g \]of food intake measured by food diary
fiber whole day (24hours) before test, in the morning before the test fiber \[g \]of food intake measured by food diary
Body mass in the morning before the test up to 1 hour after getting up Body mass \[kg\] measured by electronic scale to the nearest 0,05 kg (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan).
Body fat in the morning before the test up to 1 hour after getting up Body fat \[%\] measured by electronic scale (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan).
lactose whole day(24hours) before test, in the morning before the test lactose \[g \]of food intake measured by food diary
saccharose whole day (24hours) before test, in the morning before the test saccharose \[g \]of food intake measured by food diary
Lean body mass in the morning before the test up to 1 hour after getting up Lean body mass \[kg\] measured by electronic scale (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan).
Total body water in the morning before the test up to 1 hour after getting up Body fat \[%\] measured by electronic scale (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan).
height in the morning before the test up to 1 hour after getting up \[cm\] measured by high meter
self reported gastrointestinal scale up to 1 hours after the test 10 point scale
Trial Locations
- Locations (1)
Poznan University of Physical Education, Faculty of Sport Sciences in Gorzow
🇵🇱Gorzów Wielkopolski, Lubuskie, Poland