The Association Between Food Timing and Composition on Gastrointestinal Injury, Stress Hormone and Gastrointestinal Complains in Elite Rowers After 2000m Ergometer Test - an Observational Study.
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 13
- 试验地点
- 1
- 主要终点
- Corisol
研究概览
简要总结
The study aimed to check the influence of timing and composition of diet on markers of gut injury, endotoxemia and stress.
详细描述
The research protocol includes detailed food intake analysis, including timing and composition, markers related to the neuroendocrine pathway cortisol, and observations of individually experienced symptoms. Moreover, the study also explores intestinal injury and endotoxemia parameters, such as I-FABP, CLDN-3, and LBP. As a first goal, this study investigates the timing and composition of food intake concerning changes in gut integrity, cortisol levels, and gastrointestinal symptoms. Investigators explored whether an athlete's nutritional intake influences any GI disturbance induced by high-intensity exercise. As a second goal, Investigators tried to connect symptoms and markers from both pathways with gastrointestinal complaints. Investigators hypothesised that cortisol levels would be more profound than other studied parameters in gastrointestinal symptoms.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Retrospective
入排标准
- 年龄范围
- 18 Years 至 23 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Membership in Polish Rowing Team,
- •minimum five years of training,
- •total training time minimum of 240 minutes,
- •filling out a food diary,
- •finishing 2000-meter ergometer test.
排除标准
- •Probiotics within the last three months,
- •prebiotics within the last three months
- •antibiotic therapy within the last three months,
- •dietary regime,
- •gastrointestinal diseases,
- •lactose intolerance
结局指标
主要结局
Corisol
时间窗: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test, and 1-hour recovery period
Stress hormoneconcentration measured in blood \[ng/ml\] using commercially available enzyme-linked immunosorbent assays
I-FABP (intestinal fatty acid binding protein)to measure epithelial wall injury
时间窗: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test, and 1-hour recovery period
I-FABP concentration measured in blood \[ng/ml\] using commercially available enzyme-linked immunosorbent assays
Claudin to measure tight junction leakage
时间窗: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test, and 1-hour recovery period
Zonulin concentration measured in blood \[ng/ml\] using commercially available enzyme-linked immunosorbent assays
LBP (lipopolysaccharide binding protein) to measure endotoxin
时间窗: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test, and 1-hour recovery period
LBP concentration measured in blood \[ug/ml\] using commercially available enzyme-linked immunosorbent assays
Lactic acid to measure fatigue after the race
时间窗: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test
Lactate concentration measured in capillary blood from earlobe \[mmol/L\] using a commercially available kit (Diaglobal, Berlin, Germany)
次要结局
- protein(whole day (24hours) before test, in the morning before the test)
- fat(whole day (24hours) before test, in the morning before the test)
- glucose(whole day (24hours) before test, in the morning before the test)
- energy(whole day (24hours) before test, in the morning before the test)
- carbohydrate(whole day (24hours) before test, in the morning before the test)
- fructose(whole day (24hours) before test, in the morning before the test)
- fiber(whole day (24hours) before test, in the morning before the test)
- Body mass(in the morning before the test up to 1 hour after getting up)
- Body fat(in the morning before the test up to 1 hour after getting up)
- lactose(whole day(24hours) before test, in the morning before the test)
- saccharose(whole day (24hours) before test, in the morning before the test)
- Lean body mass(in the morning before the test up to 1 hour after getting up)
- Total body water(in the morning before the test up to 1 hour after getting up)
- height(in the morning before the test up to 1 hour after getting up)
- self reported gastrointestinal scale(up to 1 hours after the test)