effect of ß-Hydroxybutyrate and HIIT on gene expressions of Klotho/FGF axis an inflammatio

Phase 3

• Conditions: beta hydroxybutyrate, ketone body, Klotho, FGF23, FGF21, HIIT, high intensity interval, aging.

• Registration Number: IRCT20221106056419N1
• Lead Sponsor: Shahid Beheshti University of Medical Sciences

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Results First Posted: 1900-01-01
• Last Update Posted: 7 February 2023

Recruitment & Eligibility

• Status: Complete
• Sex: All
• Target Recruitment: 30

Inclusion Criteria

BMI between 18.5 to 30
not to have regular physical activity more than 1h and 3 days a week.
not to lose or gain weight more than 5kg in the last 3 months.
not to pregnant
not to have breastfeeding

Exclusion Criteria

to have chronic and acute inflammatory and infectious diseases
to have high blood pressure
to have cardiovascular diseases
to have diabetes or use diabetes medication
alcohol consumption
use thyroid medication

Study & Design

• Study Type: interventional
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
The effect of HIIT and beta-hydroxybutyrate intervention on Klotho gene expression in Peripheral Blood Mononuclear Cells(PBMC). Timepoint: Determination of Klotho gene expression level in Peripheral Blood Mononuclear Cells (PBMC) at the beginning of the intervention and 45 minutes after the intervention. Method of measurement: real time PCR.

Secondary Outcome Measures

Name	Time	Method
1. Determining the effect of HIIT and beta-hydroxybutyrate intervention on FGF23 gene expression in peripheral blood mononuclear cells. 2.Determining the effect of HIIT and beta-hydroxybutyrate intervention on FGF21 gene expression in peripheral blood mononuclear cells. 3.Determining the effect of HIIT and beta-hydroxybutyrate intervention on hsCRP plasma level. Timepoint: Examination of all outcomes at the beginning of the intervention and 45 minutes after the intervention. Method of measurement: Real-time PCR method is used to determine FGF23 and FGF21 gene expression, and biochemical kit is used to determine hsCRP plasma level.