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A study of the in-vitro thermal stability of the ryanodine receptor-1 in patients with RYR1 variants

Recruiting
Conditions
Muscle breakdown
rhabdomyolysis
10028396
Registration Number
NL-OMON50003
Lead Sponsor
Canisius Wilhelmina Ziekenhuis
Brief Summary

Not available

Detailed Description

Not available

Recruitment & Eligibility

Status
Recruiting
Sex
Not specified
Target Recruitment
20
Inclusion Criteria

Inclusion criteria RYR1 patients
* Genetically proven RYR1 variant
* Indication for an IVCT in the context of routine clinical care
* Age 18 * 65 years old

Inclusion critera healthy controls
* Indication for surgery of the upper leg in the context of routine clinical
care, e.g. removal of osteosynthesis material or elective orthopaedic surgery
of the upper leg.
* Age 18 * 65 years old

Exclusion Criteria

Exclusion criteria RYR1 patients
* Another family member is included in the study
* An MH reaction, EHS, ER, compartment syndrome, ischaemia of the lower
extremities, trauma of the upper legs or rhabdomyolysis due to another
aetiology in the past 4 months.
* A viral infection in the past week
* Other neuromuscular disorders than RYR1 related MH/ER/EHS
* Patients using systemic corticosteroids during the last 3 months
* Pregnancy or lactating
* No written informed consent by the patient

Exclusion criteria healthy controls
* A history of a RYR1 variant
* Another family member is included in the study
* A history or a family history of a RYR1 related disease (MH, EHS, ER, central
core disease, multi-minicore disease)
* A history of a neuromuscular disorder
* Patients using systemic corticosteroids during the last 3 months

* Patients with a current compartment syndrome, planned for fasciotomy
* Patients with current ischaemia of the lower extremities with an indication
for vascular surgery or amputation.
* Patients with current trauma of the upper legs and an indication for surgery.
* A compartment syndrome, ischaemia of the lower extremities, trauma of the
upper legs or rhabdomyolysis due to another aetiology in the past 4 months.
* A viral infection in the past week.
* Pregnancy or lactating
* No written informed consent by the patient

Study & Design

Study Type
Observational invasive
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
<p>The primary endpoint of the study is the number of positive IVCT*s in the<br /><br>extended protocol in the RYR1 group compared to the healthy control group. </p><br>
Secondary Outcome Measures
NameTimeMethod
<p>Secondary endpoints of the study are the concentration of caffeine and<br /><br>halothane leading to a pathological contracture in the extended IVCT in the<br /><br>RYR1 group compared to the healthy controls. Other secondary endpoints are the<br /><br>results of a questionnaires study focused on neuromuscular and heat intolerance<br /><br>complains during exercise and a family history of EHS.</p><br>
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