Role of Colonic Short Chain Fatty Acids in Obesity

Not Applicable

Completed

• Conditions: Obesity
• Interventions: Other: Glucose; Other: Inulin; Other: Resistant Starch

• Registration Number: NCT02562014
• Lead Sponsor: University of Toronto

Brief Summary

The excess production of colonic short-chain-fatty-acids (SCFA) has been implicated in the promotion of obesity, but colonic fermentation of dietary-fiber to SCFA may also play a role in preventing diabetes. The investigators aimed to compare the effects of two fermentable fibers (inulin and resistant-starch) on postprandial SCFA, glucose, insulin, free-fatty acids (FFA) and gut hormone responses and to compare the responses in healthy overweight and obese (OWO) vs lean (LN) participants. Methods: Using a randomized, single blind, crossover design, 13 OWO and 12 LN overnight fasted participants were studied on 3 separate occasions. On each day they consumed a 300 mL drink containing 75g glucose (Control) or 75g glucose plus 24g inulin (IN), or 28.2g resistant-starch (RS). A standard lunch was served 4 h after the test drink.

Detailed Description

Not available

Study Timeline

• Study Start: 2012-02
• Primary Completion: 2012-07
• Study Completion: 2013-01
• Status Verified: 2015-09
• Study First Submitted: 2015-09-25
• Study First Submitted QC: 2015-09-28
• Last Update Submitted: 2015-09-28
• Study First Posted: 2015-09-29
• Last Update Posted: 2015-09-29

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 25

Inclusion Criteria

• non-pregnant
• body mass index >=20 and <=35

Exclusion Criteria

• history of diabetes mellitus
• history of cardiovascular disease
• history of bowel disease
• history of kidney disease
• history of liver disease
• use of antibiotics within 3 months of enrolment
• unusual dietary habits
• fasting glucose >6.9mmol/L
• hematocrit below normal range for age and sex

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
Overweight	Glucose	Participants with body mass index \>25
Overweight	Inulin	Participants with body mass index \>25
Lean	Resistant Starch	Participants with body mass index \<=25
Lean	Glucose	Participants with body mass index \<=25
Overweight	Resistant Starch	Participants with body mass index \>25
Lean	Inulin	Participants with body mass index \<=25

Primary Outcome Measures

Name	Time	Method
Incremental area under the serum acetate response curve	For 6 hours after consuming the test meal

Secondary Outcome Measures

Name	Time	Method
Incremental area under the breath hydrogen response curve	For 6 hours after consuming the test meal
Incremental area under the serum propionate response curve	For 6 hours after consuming the test meal
Incremental area under the breath methane response curve	For 6 hours after consuming the test meal
Incremental area under the serum insulin response curve	For 2 hours after consuming the standard lunch
Incremental area under the serum butyrate response curve	For 6 hours after consuming the test meal
Incremental area under the serum c-peptide response curve	For 2 hours after consuming the standard lunch
Incremental area under the serum glucose response curve	For 2 hours after consuming the standard lunch
Total area under the serum free-fatty acid response curve	For 2 hours after consuming the standard lunch