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Recovery Kinetics Following Change of Direction Training

Not Applicable
Completed
Conditions
Skeletal Muscle Damage
Interventions
Other: COD45
Other: COD90
Registration Number
NCT04795232
Lead Sponsor
University of Thessaly
Brief Summary

This study aims at investigating the recovery kinetics of skeletal muscle damage, neuromuscular fatigue and performance following a single change of direction (COD) training session in competitive soccer players. The impact of COD degrees will be also examined by comparing a 45o vs. 90o COD training session. Ten male soccer players will perform a COD45 \[A single training session including 2x(10x \~27.6m) sprints with two 45o COD in each sprint\], a COD90 \[A single training session including 2x(10x \~21.2m) sprints with two 90o COD in each sprint\] and a Control trial (No intervention included, only daily measurements) in randomized, repeated measures, crossover design. Assessments related to skeletal muscle damage, neuromuscular fatigue and performance will be performed prior to training session and daily for three consecutive days post-training, in each trial.

Detailed Description

Ten male soccer players will be included in this study. Participants will be initially informed about the aim of study as well as the associated risks and benefits and subsequently will provide their signed consent form. At baseline, they will undergo assessment of their anthropometrics (i.e. body mass and body height), body composition (by DXA), physical conditioning status \[maximal oxygen consumption (VO2max), Yo-Yo Intermittent Endurance test level 2 and Yo-Yo Intermittent Recovery test level 2\] and daily dietary intake. Thereafter, they will participate in two experimental trials and one control trial in a randomized, crossover, repeated measures design: i) COD45: Participants in this trial will perform a COD training session consisted of 2 sets of 10 x \~27.6m sprints with two 45o COD in each sprint and a resting period of 16 sec and 2:20 min between sprints and sets, respectively, ii) COD90: Participants in this trial will perform a COD training session consisted of 2 sets of 10 x \~21.2m sprints with two 90o COD in each sprint and a resting period of 16 sec and 2:20 min between sprints and sets, respectively, iii) Control: Participants in this trial will only participate in daily assessments to control for day to day variability in the depended variables (they will not receive any intervention). Prior to each trial, participants will provide a resting blood sample (for the determination of blood lactate, white blood cell count and creatine kinase activity) and undergo assessment of their delayed onset of muscle soreness (DOMS), maximal voluntary isometric contraction (MVIC), countermovement jump \[will be assessed using two force platforms at 1000 Hz and electromyography instrumentation (EMG)\], isokinetic peak torque of knee extensors and flexors (will be assessed on an isokinetic dynamometer), agility (will be assessed using the Illinois test), 10m and 30m sprint time (will be assessed by using light cells) and repeated sprint ability (5x30m sprints will be performed with 25 sec rest in-between). In COD45 and COD90 experimental trials, a blood sample will be collected immediately post-training for the determination of blood lactate concentration while assessment of DOMS, MVIC and countermovement jump will be performed at 1, 2 and 3 hours post-training. In all trials assessment of DOMS, MVIC, countermovement jump (CMJ), isokinetic peak torque, agility and sprinting performance as well as determination of creatine kinase activity and white blood cell count will be performed at 24, 48 and 72 hours post-training. A 7-day wash out period will applied between trials.

Recruitment & Eligibility

Status
COMPLETED
Sex
Male
Target Recruitment
10
Inclusion Criteria
  • Soccer players participating in at least 5 training sessions per week and 1official match
  • Participation at a competitive level for at least 4 years
  • Free of musculoskeletal injuries
  • No use of ergogenic supplements or medication
  • Non-smokers
Exclusion Criteria
  • Musculoskeletal injury
  • Use of alcohol, caffeine and any type of ergogenic supplements or medication during the course of the study

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Arm && Interventions
GroupInterventionDescription
COD45COD45Participants in this arm will participate in a COD training session consisted of 45 degrees changes of direction
COD90COD90Participants in this arm will participate in a COD training session consisted of 90 degrees changes of direction
Primary Outcome Measures
NameTimeMethod
Change in white blood cell countAt baseline, at 24, 48 and 72 hours after change of direction training session

White blood cell count will be measured by using an automatic blood analyzer

Change in heart rate during the change of direction training sessionDuring the change of direction training session

Heart rate will be continuously monitored during the change of direction training session by using heart rate monitors.

Change in peak power during countermovement jumpAt baseline, at 1, 2, 3, 24, 48 and 72 hours after change of direction training session

Peak power will be assessed using two force platforms at 1000 Hz, with each foot in parallel on the two platforms providing a separate yet time-synchronized measurement of the jump height for each leg

Change in isokinetic peak torque of knee flexorsAt baseline, at 24, 48 and 72 hours after change of direction training session

Isokinetic peak torque will be assessed on an isokinetic dynamometer in both limbs

Change in countermovement jump heightAt baseline, at 1, 2, 3, 24, 48 and 72 hours after change of direction training session

Countermovement jump height will be assessed by using two force platforms at 1000 Hz, with each foot in parallel on the two platforms providing a separate yet time-synchronized measurement of the jump height for each leg

Change in peak rate of force development during countermovement jumpAt baseline, at 1, 2, 3, 24, 48 and 72 hours after change of direction training session

Peak rate of force development will be assessed using two force platforms at 1000 Hz, with each foot in parallel on the two platforms providing a separate yet time-synchronized measurement of the jump height for each leg

Change in blood lactate concentrationAt baseline and immediately post change of direction training session

Blood lactate will be measured using a lactate plus system

Change in ground reaction force during countermovement jumpAt baseline, at 1, 2, 3, 24, 48 and 72 hours after change of direction training session

Ground reaction force will be assessed by using two force platforms at 1000 Hz, with each foot in parallel on the two platforms providing a separate yet time-synchronized measurement of the jump height for each leg

Change in mean power during countermovement jumpAt baseline, at 1, 2, 3, 24, 48 and 72 hours after change of direction training session

Mean power will be assessed using two force platforms at 1000 Hz, with each foot in parallel on the two platforms providing a separate yet time-synchronized measurement of the jump height for each leg

Change in vertical stiffness during countermovement jumpAt baseline, at 1, 2, 3, 24, 48 and 72 hours after change of direction training session

Vertical stiffness will be assessed using two force platforms at 1000 Hz, with each foot in parallel on the two platforms providing a separate yet time-synchronized measurement of the jump height for each leg

Change in sprint time of 10mAt baseline, at 24, 48 and 72 hours after change of direction training session

10m sprint time will be assessed using light cells

Change in peak normalized electromyography (EMG) during the eccentric and concentric phases of the countermovement jumpAt baseline, at 1, 2, 3, 24, 48 and 72 hours after change of direction training session

EMG data will be collected wirelessly at 2000 Hz using a Myon MA-320 EMG system (Myon AG, Switzerland) for the vastus lateralis, biceps femoris, gastrocnemius and gluteus maximus muscles.

Change in sprint time of 30mAt baseline, at 24, 48 and 72 hours after change of direction training session

30m sprint time will be assessed using light cells

Change in delayed onset of muscle sorenessAt baseline, at 1, 2, 3, 24, 48 and 72 hours after change of direction training session

Muscle soreness will be assessed by palpation of the muscle belly and the distal region

Change in isometric peak torqueAt baseline, at 1, 2, 3, 24, 48 and 72 hours after change of direction training session

Isometric peak torque will be assessed on an isokinetic dynamometer

Change in isokinetic peak torque of knee extensorsAt baseline, at 24, 48 and 72 hours after change of direction training session

Isokinetic peak torque will be assessed on an isokinetic dynamometer in both limbs

Change in creatine kinase activityAt baseline, at 24, 48 and 72 hours after change of direction training session

Creatine kinase activity will be measured in plasma

Change in fatigue index of maximal voluntary isometric contraction during 10 secondsAt baseline, at 1, 2, 3, 24, 48 and 72 hours after change of direction training session

Fatigue rate will be determined by calculating the percent drop of peak torque between the first and the last three seconds of a 10-second maximal isometric contraction

Change in mean normalized electromyography (EMG) during the eccentric and concentric phases of the countermovement jumpAt baseline, at 1, 2, 3, 24, 48 and 72 hours after change of direction training session

EMG data will be collected wirelessly at 2000 Hz using a Myon MA-320 EMG system (Myon AG, Switzerland) for the vastus lateralis, biceps femoris, gastrocnemius and gluteus maximus muscles.

Change in repeated sprint abilityAt baseline, at 24, 48 and 72 hours after change of direction training session

Repeated sprint ability will be tested using 5x30 m sprints with 25 seconds rest in-between

Change in agilityAt baseline, at 24, 48 and 72 hours after change of direction training session

Agility will be assessed using the illinois agility test.

Change in field activity during the change of direction training sessionDuring the change of direction training session

Field activity will be continuously monitored during the change of direction training session by using global positioning system (GPS)

Secondary Outcome Measures
NameTimeMethod
Yo-Yo Intermittent Endurance Test level 2 performanceAt baseline
Body fatAt baseline

Body fat will be assessed by using Dual-emission X-ray absorptiometry

Body heightAt baseline

Body height will me measured on a beam balance with stadiometer

Yo-Yo Intermittent Recovery Test level 2 performanceAt baseline
Lean body massAt baseline

Lean body mass will be assessed by using Dual-emission X-ray absorptiometry

Dietary intakeAt baseline

Dietary intake will be assessed over a 7-day period by using diet recalls

Body massAt baseline

Body mass will me measured on a beam balance with stadiometer

Maximal oxygen consumption (VO2max)At baseline

VO2max will be measured by open circuit spirometry via breath by breath method

Trial Locations

Locations (2)

SMART LAB, Department of Physical Education and Sports Science, University of Thessaly.

🇬🇷

Trikala, Greece

SmArT LABORATORY, SCHOOL OF PHYSICAL EDUCATION & SPORTS SCIENCES, UNIVERSITY OF THESSALY

🇬🇷

Trikala, Greece

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