The Effects of Acute Nutritional Ketosis on Myofibrillar Protein Synthesis in Young Men
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 36
- 试验地点
- 2
- 主要终点
- Fractional synthetic rate of muscle protein synthesis (myofibrillar)
研究概览
简要总结
Ketones are natural substances normally produced by the body during prolonged fasting and starvation, or in response to a "ketogenic" diet to be used as fuel by the brain and muscles. Ketones are therefore similar to dietary proteins, carbohydrates and fats since they represent a source of energy for the body. In addition to serving as a source of energy, ketones have also been shown to stimulate increased rates of muscle protein synthesis in humans.
The ingestion of dietary protein is well established to stimulate an increase in the rate of protein synthesis in skeletal muscle. The rate of muscle protein synthesis can be maximized following the intake of 20g of protein. As a result, smaller doses of protein (i.e. 10g) represent a sub-optimal dose of protein because there is still room for improvement concerning muscle protein synthesis.
Recently ketone-containing food products have become available that elevate ketone levels in the body without the need for ketogenic diets or prolonged fasting. Therefore, the purpose of this study is to measure skeletal muscle protein synthesis rates after ingesting the following:
- Ketone monoester
- Ketone monoester supplemented with sub-optimal dose of whey protein
- Sub-optimal dose of whey protein
It is hypothesized that muscle protein synthesis rates will increase following the ingestion of a ketone-containing beverage. Further, muscle protein synthesis rates will be enhanced when the ketone-containing beverage and sub-optimal protein dose are taken together.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Treatment
- 盲法
- Quadruple (Participant, Care Provider, Investigator, Outcomes Assessor)
入排标准
- 年龄范围
- 18 Years 至 35 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Aged between 18-35 years inclusive
- •Healthy, moderately active
- •BMI < 30 kg/m2 and > 18.5 kg/m2
- •Having given informed consent
排除标准
- •Presence of any identified metabolic or intestinal disorders
- •Use of tobacco products
- •Allergies to milk proteins (whey or casein)
- •Lactose intolerance
- •Phenylketonuria (PKU)
- •A history of neuromuscular problems
- •Previous participation in amino acid tracer studies
- •Adherence to a strict vegetarian or vegan diet
- •Current use of ketone supplements or adherence to a ketogenic diet
- •Use of medications known to affect protein metabolism
- •Diagnosis of Diabetes
- •Engagement in sports or physical exercise 5 or more days per week
结局指标
主要结局
Fractional synthetic rate of muscle protein synthesis (myofibrillar)
时间窗: 0-5 hours in the post-prandial period
次要结局
- Fractional synthetic rate of muscle protein synthesis (myofibrillar)(0-3 hours in the pre-prandial period; 0-2 hours, and 2-5 hours into the post-prandial period)
- Plasma enrichments (in moles percent excess) of L-[ring-2H5]-phenylalanine(3 hours pre-prandial to 5 hours post-prandial)
- Plasma insulin concentration (pmol/L)(3 hours pre-prandial to 5 hours post-prandial)
- Plasma amino acid concentrations (mmol/L)(3 hours pre-prandial to 5 hours post-prandial)
- Signaling molecule phosphorylation status(0, 2, and 5 hours into the post-prandial period)
- Plasma glucose concentration (mmol/L)(3 hours pre-prandial to 5 hours post-prandial)
- Capillary blood Beta-OHB concentrations (mmol/L)(0-5 hours in the post-prandial period)
研究者
Tyler Churchward-Venne
Tyler A. Churchward-Venne, Ph.D., Assistant Professor, McGill University
McGill University