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The effect of cold acclimation on brown adipose tissue.

Completed
Conditions
Brown adipose tissueCold acclimationSkeletal muscleNon-shivering thermogenesis
Registration Number
NL-OMON20696
Lead Sponsor
Maastricht University Medical Centre + (MUMC+)
Brief Summary

/A

Detailed Description

Not available

Recruitment & Eligibility

Status
Completed
Sex
Not specified
Target Recruitment
20
Inclusion Criteria

1. BMI 18-25;

2. Age 18-30;

Exclusion Criteria

1. Diabetes Mellitus;

2. Females: Pregnancy;

Study & Design

Study Type
Interventional
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
1. Standard uptake values (SUVs) of FDG of active brown adipose tissue with the PET/CT-scans, one time prior and one time post the cold acclimation period (2x 2hour);<br /><br>2. Energy expenditure with indirect calorimetry, one time prior, half way and one time post the cold acclimation period (3x 3hour);<br /><br>3. Skeletal muscle mitochondrial uncoupling with the muscle biopsy, one time before and one time post the cold acclimation period (2x 30minutes). <br>BRITE cell recruitment with the fat biopsy, one time prior and one time post the cold acclimation period (2x 30minutes).
Secondary Outcome Measures
NameTimeMethod
1. Body temperatures (core and skin temperatures) with iButtons and a telemetric pill, one time prior, half way and one time post the cold acclimation period;<br /><br>2. Skin perfusion with laser Doppler flowmetry, one time prior, half way and one time post the cold acclimation period;<br /><br>3. Blood parameters, among others insulin, glucose, free fatty acids and thyroid hormones, one time prior, half way and post the cold acclimation period;<br /><br>4. Body composition with a DXA-scan, one time prior and post the cold acclimation period.
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