Serum Exosomal Long Noncoding RNAs as Potential Biomarkers for Lung Cancer Diagnosis
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 1,000
- 试验地点
- 1
- 主要终点
- the CT scans of the lung for the patients
研究概览
简要总结
The study is to investigate the sensitivity and specificity of serum exosome noncoding RNA as a biomarker for the diagnosis of lung cancer
详细描述
Conventional tumor markers for non-invasive diagnosis of Lung cancer (LC) exhibit insufficient sensitivity and specificity to facilitate detection of early lung cancer (ELC). The investigators aimed to identify ELC-specific exosomal lncRNA biomarkers that are highly sensitive and stable for the non-invasive diagnosis of ELC.Hence, in the present study, exosomes from the plasma of five healthy individuals and 30 LC patients and from culture media of four human bronchial epithelial cells and four cancer cells were isolated. Exosomal RNA profiling was performed using RNA sequencing to identify LC specific exosomal lncRNAs.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 75 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Subjects are willing to sign the informed consent form;
- •Normal subjects have no evidence of any disease;
- •Lung cancer patients are 18 to 75 years old, diagnosed as lung cancer by histopathology or cytopathology
排除标准
- •People are unwilling to sign the informed consent form;
- •Patients with heart disease, rheumatic disease, allergic disease, COPD, pulmonary fibrosis, diabetes, thyroid disease, liver, kidney, brain disease and hematopoietic system disease;
- •Pregnant or lactating women;
- •Patients did not cooperate or participate in other clinical trials
结局指标
主要结局
the CT scans of the lung for the patients
时间窗: the first day subjects are enrolled the outcome will be assessed；up to three years after the completion of the study
The expression levels of serum exosome long non-coding RNA
时间窗: the first day subjects are enrolled the outcome will be assessed；up to three years after the completion of the study
the expression levels of tumor biomarkers such as CEA, NSE, SCC, CYFR2A-1
时间窗: the first day subjects are enrolled the outcome will be assessed；up to three years after the completion of the study
次要结局
未报告次要终点
研究者
Yang Jin
professor
Wuhan Union Hospital, China