Evaluation of sweet taste receptors, glucose transporters, glucose absorption and gastrointestinal hormones in obesity and after bariatric surgery.

Not Applicable

Recruiting

• Conditions: Obesity; Laparoscopic adjustable gastric banding (LAGB) and Roux-en-Y Gastric Bypass (RYGB) surgery; Diabetes Mellitus; Diet and Nutrition - Obesity; Surgery - Other surgery; Oral and Gastrointestinal - Normal oral and gastrointestinal development and function

• Registration Number: ACTRN12613000758707
• Lead Sponsor: Dr. Nam Nguyen

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Results First Posted: 1900-01-01
• Study Start: 2013-07-08
• Last Update Posted: 13 January 2020

Recruitment & Eligibility

• Status: Recruiting
• Sex: All
• Target Recruitment: 90

Inclusion Criteria

Obese patients - BMI > 40kg/m2
Bariatric patients - RYGB or LAGB surgery > 12 months ago
Healthy volunteers - BMI < 40kg/m2

Exclusion Criteria

History of severe respiratory, cardiovascular, hepatic and/or renal disease, chronic alcohol abuse or epilepsy (excluded by history)
Medication that may influence gastrointestinal function
History of surgery to the gastrointestinal tract apart from the RYGB or LAGB
Female patients not using appropriate contraceptive method (ie oral contraceptive pill, diaphragm, Depo-Provera hormonal contraceptive injection, intrauterine device, Norplant method)
Pregnant and/or breastfeeding mothers

Study & Design

• Study Type: Interventional
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Small intestinal sweet taste receptors (T1R2, T1R3, Gagust and TRPM5) during fasting and following 30 minutes of glucose stimulation measured by biopsies taken during endoscopy[3 biopsies taken at t=0min and 30min];Small intestinal carbohydrate transporters (SGLT1 and GLUT2) during fasting and following 30 minute of glucose stimulation as measured by biopsies taken at various timpoints during endoscopy.[3 biopsies taken at t=0min and 30min];Glucose absorption (measured using 3-OMG concentrations (from blood sample) area under curves 0-240 min)[blood samples taken at t = 0, 15, 30, 45, 60, 75, 90, 105, 120, 150, 180, 210, and 240 mins. ]

Secondary Outcome Measures

Name	Time	Method
The relationships between sweet taste receptors, carbohydrate transporters, glucose absorption and release of incretin hormones (GLP-1, PYY, CCK and insulin).[This is analysed after all data collection is complete]