Role of the Gut Sweet Taste Receptor in the Secretion of Satiation Peptides

Phase 1

Completed

• Conditions: Taste Disorder, Secondary, Sweet
• Interventions: Dietary Supplement: lactisole

• Registration Number: NCT01302574
• Lead Sponsor: University Hospital, Basel, Switzerland

Brief Summary

The purpose of this study is to determine the functional significance of sweet taste receptors in the secretion of gastrointestinal (GI) satiation peptides by using a specific sweet taste receptor antagonist to block sweet taste perception in the gastrointestinal tract.

Detailed Description

There is strong evidence that taste signaling mechanisms identified in the oral epithelium also operate in the gut. It is suggested that open-type enteroendocrine cells directly sense nutrient via alpha-gustducin coupled taste receptors to modulate the secretion of glucagon like peptide-1 (GLP-1) and peptide YY (PYY). Several nutrient responsive G-protein coupled receptors have been identified in the human gut, including the sweet taste responsive T1R2/T1R3 heterodimer, the amino acid/umami responsive T1R1/T1R3 as well as GPR120 for unsaturated long-chain free fatty acids. The functional significance of sweet taste receptors in mixed liquid meal-stimulated secretion of GLP-1 and PYY will be determined by intragastric infusion of a 500 mL mixed liquid meal with or without lactisole (450 ppm)in a double blind, 2-way crossover trial including 16 healthy subjects.

Study Timeline

• Study Start: 2010-01
• Primary Completion: 2010-03
• Study Completion: 2010-06
• Status Verified: 2011-02
• Study First Submitted: 2011-02-22
• Study First Submitted QC: 2011-02-23
• Last Update Submitted: 2011-02-23
• Study First Posted: 2011-02-24
• Last Update Posted: 2011-02-24

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 16

Inclusion Criteria

• healthy subjects
• BMI of 19.0-24.5
• age 18-40 years old
• stable body weight for at least 3 month

Exclusion Criteria

• smoking
• substance abuse
• regular intake of medication
• medical or psychiatric illness
• gastrointestinal disorders or food allergies

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
mixed liquid meal	lactisole	500 mL mixed liquid meal + 50 mg 13C-sodium-acetate
mixed liquid meal + lactisole	lactisole	500 mL mixed liquid meal + 50 mg 13C-sodium-acetate + 450 ppm lactisole

Primary Outcome Measures

Name	Time	Method
Gastrointestinal peptide secretion	2 hours blood sampling

Secondary Outcome Measures

Name	Time	Method
Appetite perceptions	4 hours
Gastric emptying rate	4 hours

Trial Locations

Locations (1)

University Hospital Basel, Phase 1 Research Unit; 🇨🇭 Basel, Switzerland