SEGATROM: Sensory and Gastrointestinal Impact of Taste Receptor Variants on Human Metabolism and Nutrition

Not Applicable

Completed

• Conditions: Disorder of Glucose Regulation
• Interventions: Dietary Supplement: sweet block; Other: gastroscopy; Dietary Supplement: bitter block; Dietary Supplement: umami-block

• Registration Number: NCT02219295
• Lead Sponsor: German Institute of Human Nutrition

Brief Summary

We investigate the impact of taste receptors (sweet, bitter and umami) on human metabolism. There ist growing evidence, that components of the taste signalling system, including the taste receptor proteins , have important functions in the regulation of metabolic parameters in non-gustatory tissues.

Detailed Description

We apply different sweet, bitter and umami taste compounds in a single dose in the human intervention. We use substances as for instance glucose, sucrose,saccharin and lactisol in the sweet part, and substances from cabbage, beer or red endive in the bitter part. For umami we take glutamate.

Blood samples are collected at baseline and 6 times thereafter up to 3hours, to analyse the incretins GLP-1(glucagon-like peptide 1) and GIP(gastrointestinal polypeptide) and other peptide hormones as insulin and PYY(peptide tyrosin trosin) in human beings.

Gastrointestinal mucosa is taken by gastroscopy from the lower duodenum from study subjects for analysis for the presence or absence of functional taste receptors in gastrointestinal tissues.

Study Timeline

• Study Start: 2011-12
• Study First Submitted: 2014-08-01
• Study First Submitted QC: 2014-08-15
• Study First Posted: 2014-08-18
• Primary Completion: 2014-09
• Study Completion: 2014-09
• Status Verified: 2020-06
• Last Update Submitted: 2020-06-23
• Last Update Posted: 2020-06-24

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 97

Inclusion Criteria

• Healthy persons

Exclusion Criteria

• Tumor in history
• Pregnancy
• Severe illness in history
• Bodymass index below 18

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: FACTORIAL

Arm && Interventions

Group	Intervention	Description
sweet -block	umami-block	application of different sugars, artificial sweeteners and sweet-taste antagonists in a single dose in 300 ml tap water every week up to 7 times and collection of blood samples over 3 hours
sweet -block	bitter block	application of different sugars, artificial sweeteners and sweet-taste antagonists in a single dose in 300 ml tap water every week up to 7 times and collection of blood samples over 3 hours
bitter block	bitter block	application of purified bitter tasting ingredients of vegetables and hop in 300 ml tap water , collection of blood samples for 3h (-15,0,15,30,60,120,180 min)
sweet -block	sweet block	application of different sugars, artificial sweeteners and sweet-taste antagonists in a single dose in 300 ml tap water every week up to 7 times and collection of blood samples over 3 hours
bitter block	umami-block	application of purified bitter tasting ingredients of vegetables and hop in 300 ml tap water , collection of blood samples for 3h (-15,0,15,30,60,120,180 min)
umami-block	sweet block	application of glutamate and glutamate +IMP same procedure as above
umami-block	bitter block	application of glutamate and glutamate +IMP same procedure as above
umami-block	umami-block	application of glutamate and glutamate +IMP same procedure as above
bitter block	sweet block	application of purified bitter tasting ingredients of vegetables and hop in 300 ml tap water , collection of blood samples for 3h (-15,0,15,30,60,120,180 min)
gastroscopy	gastroscopy	gastroscopy with and without oral intervention with artificial sweetener saccharin to take tissue samples from the upper bowel for the investigation of taste receptor cells in the upper bowel in human beings

Primary Outcome Measures

Name	Time	Method
Area under the curve (AUC): changes of expression levels of the sweet , bitter und umami taste receptors in humans in the upper bowel and responses of incretins to receptor agonists and antagonists	three years	measurement of incretins (GIP, GLP-1) and peptide hormones in human plasma after consumption of different sugars , sweeteners , bitter tasting ingredients of vegetables und umami. Measurement of expression levels in human intestinal mucosa before and after receptor stimulation

Secondary Outcome Measures

Name	Time	Method
changes in the signalling cascade of the sweet receptor SGLT1 (sodium tranporter 1), changes of receptor expression	3 years	oral application of sweet tasting substances and antagonists, endoscopic collection of tissue samples, determination of molecular resonses by qRT-PCR and immunohistochemistry

Trial Locations

Locations (1)

Department of Clinical Nutrition; 🇩🇪 Bergholz-Rehbrücke, Brandenburg, Germany