Harnessing Macrophage Lysosomal Lipid Metabolism in Obesity (ATM)
- Conditions
- Diabetes Mellitus, Type 2Non-Alcoholic Fatty Liver DiseaseObesityHealthy
- Registration Number
- NCT06571474
- Lead Sponsor
- Bettina Mittendorfer
- Brief Summary
The goal of this study is to evaluate the role of transcription factor EB (TFEB) in adipose (fat) tissue macrophages (ATM) in regulating adipose tissue and systemic metabolic function in obesity. The investigators will assess the differences in ATM lipid metabolism in people with metabolically abnormal obesity and lean individuals.
Both groups will have:
* screening visit
* imaging (body composition testing - dual-energy x-ray absorptiometry (DEXA) scans, magnetic resonance imaging \[MRI\] and magnetic resonance spectroscopy \[MRS\] scans)
* Overnight visit with intravenous infusion (IV), muscle, and fat tissue biopsies
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- RECRUITING
- Sex
- All
- Target Recruitment
- 60
Not provided
- Individuals that do not meet all inclusion Criterion
Study & Design
- Study Type
- OBSERVATIONAL
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method 24-hour plasma glucose concentration Within 2 weeks of Clinical Visit Macrophage isolation in skeletal muscle tissue Within 2 weeks of Clinical Visit macrophage gene expression using RNA sequencing and fluorescent activated cell sorting (FACS)
Insulin sensitivity Within 2 weeks of Clinical Visit Sensitivity assessed as insulin-mediated glucose disposal during a hyperinsulinemic-euglycemic clamp procedure
Macrophage isolation in adipose tissue biopsy Within 2 weeks of Clinical Visit macrophage gene expression using RNA sequencing and fluorescent activated cell sorting (FACS)
- Secondary Outcome Measures
Name Time Method
Trial Locations
- Locations (1)
University of Missouri School of Medicine
🇺🇸Columbia, Missouri, United States