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Effects of a Single Bout of Resistance Training Session on Hormonal Response in Pre-pubertal and Pubertal Boys

Not Applicable
Conditions
Hormones
Cytokines
Interventions
Other: Resistance Training
Registration Number
NCT05022992
Lead Sponsor
Umeå University
Brief Summary

: Resistance training induces strength gains in both children and adolescents. Exercise training is linked to anabolic functions through the GH-IGF-I axis. This has been demonstrated in studies showing a higher activity of GH and IGF-I in fitter adolescents and adults. Exercise also stimulates pro-inflammatory cytokines (IL-1β, IL-6, TNF-α) that suppress the GH-IGF-I axis. This has been observed in endurance-type training, while resistance training has received less attention.

Boys experience a significant physiological muscle-growth change during puberty that is associated with increases in boys androgen levels. How maturation affects the hormonal response to resistance training in boys is unclear. Therefore, the purpose of the study is to compare the acute effects of a single bout of resistance training on hormones and pro-inflammatory cytokines in pre-pubertal and pubertal boys.

Detailed Description

Preliminary measurements:

Preliminary testing will include anthropometric measurements of body mass, height, body fat percentage, and lean leg volume (LLV). The maturation stage will be determined according to the Tanner scale. There will also be an assessment of maximal strength testing to determine exercise intensity for each individual. Two familiarization sessions will be conducted for muscular strength before the muscular strength testing session.

Main trial A venous blood sample will be collected pre-exercise at resting condition. The trial will start with a standardized warm-up followed by a short (20-30 min), high-intensity whole-body resistance training session. Venous blood samples will be collected immediately after the completion of the training session and during the recovery period.

Recruitment & Eligibility

Status
UNKNOWN
Sex
Male
Target Recruitment
40
Inclusion Criteria
  • Healthy boys (n=40)
  • Physically active, defined as a minimum of 2 years experience in a sports club
  • Biological age range: 20 pre-pubertal (Tanner I-II) - 20 pubertal (Tanner III-IV) boys
  • No previous experience of structured resistance training
Exclusion Criteria
  • Chronic diseases that affect full participation
  • Injuries that limit full participation

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Resistance Training SessionResistance TrainingResistance training. Heavy resistance training. 10 reps x 3 set of upper- and lower-body resistance exercises
Primary Outcome Measures
NameTimeMethod
Growth Hormone BaselineBaseline testing before the exercise intervention (Pre values)

Blood Concentration of Growth Hormone

Change from Baseline Growth Hormone 30 minutes post exercise30 minutes after the completion of the exercise intervention (Post-30 values)

Blood Concentration of Growth Hormone

Change from Baseline Insulin-like Growth Factor-1 Post exerciseRight after the completion of the exercise intervention (Post values)

Blood concentration of Insulin-like Growth Factor-1

Change from Baseline Insulin-like Growth Factor-1 30 minutes Post exercise30 minutes after the completion of the exercise intervention (Post-30 values)

Blood concentration of Insulin-like Growth Factor-1

Change from Baseline Growth Hormone Post exerciseRight after the completion of the exercise intervention (Post values)

Blood Concentration of Growth Hormone

Change from Baseline Growth Hormone 15 minutes post exercise15 minutes after the completion of the exercise intervention (Post-15 values)

Blood Concentration of Growth Hormone

Change from Baseline Insulin-like Growth Factor-1 15 minutes Post exercise15 minutes after the completion of the exercise intervention (Post-15 values)

Blood concentration of Insulin-like Growth Factor-1

Insulin-like Growth Factor-1 BaselineBaseline testing before the exercise intervention (Pre values)

Blood concentration of Insulin-like Growth Factor-1

Secondary Outcome Measures
NameTimeMethod
Testosterone BaselineBaseline testing before the exercise intervention (Pre values)

Blood concentration of testosterone

Change from Baseline Testosterone Post ExerciseRight after the completion of the exercise intervention (Post values)

Blood concentration of testosterone

Change from Baseline Interleukin-6 15 minutes Post Exercise15 minutes after the completion of the exercise intervention (Post-15 values)

Blood concentration of Interleukin-6

Interleukin-1 alpha BaselineBaseline testing before the exercise intervention (Pre values)

Blood concentration of Interleukin-1 alpha

Sex Hormone Binding Globulin BaselineBaseline testing before the exercise intervention (Pre values)

Blood concentration of Sex Hormone Binding Globulin

Change from Baseline Baseline Sex Hormone Binding 15 minutes Post Exercise15 minutes after the completion of the exercise intervention (Post-15 values)

Blood concentration of Sex Hormone Binding Globulin

Change from Baseline Sex Hormone Binding Globulin 30 minutes Post Exercise30 minutes after the completion of the exercise intervention (Post-30 values)

Blood concentration of Sex Hormone Binding Globulin

Change from Baseline Cortisol 30 minutes Post exercise30 minutes after the completion of the exercise intervention (Post-30 values)

Blood concentration of Cortisol

Interleukin-6 BaselineBaseline testing before the exercise intervention (Pre values)

Blood concentration of Interleukin-6

Change from Baseline Interleukin-1 alpha 15 minutes Post Exercise15 minutes after the completion of the exercise intervention (Post-15 values)

Blood concentration of Interleukin-1 alpha

Change from Baseline Interleukin-1 alpha 30 minutes Post Exercise30 minutes after the completion of the exercise intervention (Post-30 values)

Blood concentration of Interleukin-1 alpha

Change from Baseline Baseline Sex Hormone Binding Post ExerciseRight after the completion of the exercise intervention (Post values)

Blood concentration of Sex Hormone Binding Globulin

Cortisol BaselineBaseline testing before the exercise intervention (Pre values)

Blood concentration of Cortisol

Change from Baseline Cortisol Post exerciseRight after the completion of the exercise intervention (Post values)

Blood concentration of Cortisol

Change from Baseline Testosterone 30 minutes Post Exercise30 minutes after the completion of the exercise intervention (Post-30 values)

Blood concentration of testosterone

Tumor Necrosis Factor alpha BaselineBaseline testing before the exercise intervention (Pre values)

Blood concentration of Tumor Necrosis Factor alpha

Change from Baseline Tumor Necrosis Factor alpha 15 minutes Post Exercise15 minutes after the completion of the exercise intervention (Post-15 values)

Blood concentration of Tumor Necrosis Factor alpha

Change from Baseline Cortisol 15 minutes Post exercise15 minutes after the completion of the exercise intervention (Post-15 values)

Blood concentration of Cortisol

Change from Baseline Testosterone 15 minutes Post Exercise15 minutes after the completion of the exercise intervention (Post-15 values)

Blood concentration of testosterone

Change from Baseline Interleukin-1 alpha Post ExerciseRight after the completion of the exercise intervention (Post values)

Blood concentration of Interleukin-1 alpha

Change from Baseline Tumor Necrosis Factor alpha Post ExerciseRight after the completion of the exercise intervention (Post values)

Blood concentration of Tumor Necrosis Factor alpha

Change from Baseline Interleukin-6 Post ExerciseRight after the completion of the exercise intervention (Post values)

Blood concentration of Interleukin-6

Change from Baseline Interleukin-6 30 minutes Post Exercise30 minutes after the completion of the exercise intervention (Post-30 values)

Blood concentration of Interleukin-6

Change from Baseline Tumor Necrosis Factor alpha 30 minutes Post Exercise30 minutes after the completion of the exercise intervention (Post-30 values)

Blood concentration of Tumor Necrosis Factor alpha

Trial Locations

Locations (1)

Umeå Movement and Exercise Laboratory, Umeå University

🇸🇪

Umeå, Västerbotten, Sweden

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