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Patients Treated for SCID (1968-Present)

Conditions
XSCID
ADA-SCID
SCID
Omenn Syndrome
Reticular Dysgenesis
Leaky SCID
Registration Number
NCT01346150
Lead Sponsor
National Institute of Allergy and Infectious Diseases (NIAID)
Brief Summary

Individuals with a past diagnosis of severe combined immune deficiency (including many cases of "leaky SCID", Omenn syndrome, and reticular dysgenesis) who have undergone blood and marrow transplant, gene therapy, or enzyme replacement in the past may be eligible for this study. The purpose of study is to look backwards at what has already been done in the. Over 800 patients with SCID are expected to be enrolled, making this one of the largest studies ever to describe outcomes for patients with SCID treated at many different hospitals around North America.

Detailed Description

One of the most important components of this study is the "cross sectional" aspect. Patients who have received their treatments (BMT, gene therapy, enzyme replacement) many years ago are asked to come back to the hospital where they were treated. During this visit, additional research bloodwork is drawn, and information is gathered regarding long-term transplant outcomes such as infections, graft-versus-host disease, autoimmune diseases, and quality of life. This will allow PIDTC researchers to better understand long-term outcomes from procedures that occurred many years ago (sometimes over 30 years ago). This will help researchers to best design new treatments and clinical trials in the future for children with SCID.

Recruitment & Eligibility

Status
UNKNOWN
Sex
All
Target Recruitment
1007
Inclusion Criteria

Strata A, B, and C (Part 1 - Retrospective Study)-

  • Individuals with Severe Combined Immune Deficiency (SCID) diagnosis who:

    --were treated at a location participating in this consortium from 1968 until present, and

    --are not enrolled in RDCRN PIDTC-6901 (ClinicalTrials.gov ID: NCT01186913).

  • Subjects who received HCT/GT/ERT prior to the present date are eligible for the retrospective study. The enrollment criteria for subjects who died prior to definitive therapy are the same as for Strata A, B and C.

Stratum A, Typical SCID:

  • Individuals who meet the following inclusion criteria and who received HCT are eligible for enrollment into Stratum A of the study:

    • Absence or very low number of T cells (CD3 T cells < 300/microliter), and no or very low T cell function (< 10% of lower limit of normal) as measured by response to phytohemagglutinin (PHA) or cells of maternal origin present.
    • If maternal cells are present but the patient does not meet criteria for very low T cell function as defined, the assigned reviewers for the potential subject, and if necessary, the full PID-SCID RP will review the laboratory report to determine if criteria of maternal engraftment are met for Protocol 6902.
    • Laboratory report of testing for maternal engraftment is required, for evaluation by the PID-SCID RP.

Stratum B, Leaky SCID, Omenn Syndrome, Reticular Dysgenesis:

Individuals who meet the following criteria are eligible for enrollment into Stratum B of the study:

Leaky SCID-

  • Maternal lymphocytes tested for and not detected and,

  • Either one or both of the following (a,b):

    a) < 50% of lower limit of normal T cell function (as measured by response to PHA OR < 50% of lower limit of normal T cell function as measured by response to CD3/CD28 antibody, b) Absent or < 30% lower limit of normal proliferative responses to candida and tetanus toxoid antigens postvaccination or exposure,

  • AND at least one of the following (a through e):

    1. Reduced number of CD3 T cells,
    2. > 80% of CD3+ or CD4 T cells are CD45RO+,
  • AND/OR >80% of CD3+ or CD4+ T cells are,CD62L negative,

  • AND/OR >50% of CD3+ or CD4+ T cells express HLA-DR (at < 4 years of age),

  • AND/OR are oligoclonal T cells. c) Hypomorphic mutation in IL2RG in a male, or homozygous hypomorphic mutation or compound heterozygosity with at least one hypomorphic mutation in an autosomal SCID-causing gene.

    d) Low TRECs and/or the percentage of CD4+/45RA+/CD31+ or CD4+/45RA+/CD62L+ cells is below the lower limit of normal.

    e) Functional testing in vitro supporting impaired, but not absent, activity of the mutant protein,

    • AND does not meet criteria for Omenn Syndrome,
    • AND does not have known selective loss of lymphocytes, Ataxia- Telangiectasia, or congenital heart defect associated with lymphopenia, unless a SCID genotype is also present.

Omenn Syndrome (OS):

  • Generalized skin rash,
  • Maternal engraftment tested for and not detected,
  • Absent or low (up to 30% of normal) T cell proliferation to antigens to which the patient has been exposed.
  • If the proliferation to antigen was not performed, but at least 4 of the following 10 supportive criteria, at least one of which must be among those marked with an asterisk (*) are present, the patient is eligible: hepatomegaly; splenomegaly; lymphadenopathy; elevated IgE; elevated absolute eosinophil count; *oligoclonal T cells measured by CDR3 length or flow cytometry >80% of CD4+ T cells are CD45RO+ ;*proliferation to PHA is reduced <50% of lower limit of normal or SI <30; *proliferative response in mixed leukocyte reaction is reduced to increment cpm < 20% or SI <20; hypomorphic mutation to SCID causing gene; low TRECs and/or percentage of CD 4+/ RA+/CD31+; or CD4+/RA+/CD62L+ cells below the lower limit of normal.

Reticular Dysgenesis (RD):

  • Absence or very low number of T cells (CD3 T cells <300/microliter),

  • No or very low (<10% of lower limit of normal) T cell function (as measured by response to phytohemagglutinin (PHA),

  • Severe congenital neutropenia (absolute neutrophil count <200/microliter),

  • AND at least one of the following:

    • Sensorineural deafness and/or absence of granulopoiesis at bone marrow examination and/or a deleterious AK2 mutation,
    • absence of granulopoiesis on bone marrow examination; a pathogenic mutation in the adenylate kinase 2 (AK2) gene identified.

Stratum C, SCID with Non-HCT Treatments:

-Individuals who meet the following criteria and were treated with PEG-ADA or gene therapy with autologous modified cells are eligible for enrollment into Stratum C (SCID with non-HCT treatments) of the study-

  • Any SCID patient previously treated with a thymus transplant (includes intention to treat with HCT, as well as PEG-ADA ERT or gene therapy).

Strata A, B, and C (Part 2 - Cross-Sectional Study):

Patient inclusion criteria for the cross sectional study: Eligibility for Strata A, B and C are the same as for the retrospective study except that all the patients in the cross-sectional study are currently surviving and are at least 2 years post the most recent class of therapy.

Exclusion Criteria

Parts 1 and 2 - Retrospective and Cross-Sectional Studies -

  • Lack of appropriate testing to rule out HIV infection after 1997 (p24 antigen or more sensitive) or other cause of secondary immunodeficiency,

  • Presence of DiGeorge syndrome,

  • Most patients with other PIDs such as nucleoside phosphorylase deficiency, ZAP70 deficiency, CD40 ligand deficiency, NEMO deficiency, XLP, cartilage hair hypoplasia or ataxia telangiectasia will not meet the inclusion criteria for Stratum A, B, or C above; however, a patient with one of the above may meet the inclusion criteria for Stratum B and if so will be included-

    • MHC Class I and MHC Class II antigen deficiency are excluded,
    • Metabolic conditions that imitate SCID or related disorders such as folate transporter deficiency, severe zinc deficiency, transcobalamin deficiency.

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
Retrospective Study - Part 11, 5, 10, 20, >20 years

Overall survival

Cross-Sectional Study - Part 22 to > 20 years

Full immune reconstitution

Secondary Outcome Measures
NameTimeMethod
Retrospective Study Part 11 year to > 20 years

Immune reconstitution and clinical outcomes

Retrospective Study - Part 13 months to >20 years

Engraftment

Cross-Sectional Study - Part 22 to >20 years

Current status of health

Trial Locations

Locations (36)

The Children's Hospital of Philadelphia

🇺🇸

Philadelphia, Pennsylvania, United States

University of Texas Southwestern Medical Center Dallas

🇺🇸

Dallas, Texas, United States

The Hospital for Sick Children (SickKids)

🇨🇦

Toronto, Ontario, Canada

Children's Hospital Boston

🇺🇸

Boston, Massachusetts, United States

Children's Hospital Denver:Center for Cancer and Blood Disorders

🇺🇸

Denver, Colorado, United States

Duke University Medical Center: Department of Pediatrics, Division of Allergy/Immunology

🇺🇸

Durham, North Carolina, United States

University of Minnesota Medical Center

🇺🇸

Minneapolis, Minnesota, United States

Oregon Health & Science University: Pediatric Hematology/Oncology

🇺🇸

Portland, Oregon, United States

Cancer Care Manitoba

🇨🇦

Winnipeg, Manitoba, Canada

Mattel Children's Hospital UCLA: Division of Pediatric Hematology/Oncology

🇺🇸

Los Angeles, California, United States

Johns Hopkins All Children's Hospital

🇺🇸

Saint Petersburg, Florida, United States

Lucile Salter Packard Children's Hospital at Stanford

🇺🇸

Palo Alto, California, United States

Memorial Sloan-Kettering Cancer Center: Department of Pediatrics

🇺🇸

New York, New York, United States

University of Alabama at Birmingham

🇺🇸

Birmingham, Alabama, United States

NIH Clinical Center Genetic Immunotherapy Section

🇺🇸

Bethesda, Maryland, United States

Alberta Children's Hospital

🇨🇦

Calgary, Alberta, Canada

Children's Hospital Los Angeles

🇺🇸

Los Angeles, California, United States

University of California San Francisco Children's Hospital

🇺🇸

San Francisco, California, United States

SSM Cardinal Glennon Children's Medical Center

🇺🇸

Saint Louis, Missouri, United States

Children's Hospital/Louisiana State University Health Sciences Center

🇺🇸

New Orleans, Louisiana, United States

University of Michigan Health System

🇺🇸

Ann Arbor, Michigan, United States

Children's Healthcare of Atlanta/Emory University School of Medicine

🇺🇸

Atlanta, Georgia, United States

Lurie Children's Hospital of Chicago

🇺🇸

Chicago, Illinois, United States

Cincinnati Children's Hospital Medical Center

🇺🇸

Cincinnati, Ohio, United States

Fred Hutchinson Cancer Research Center: Clinical Research Division and Pediatric Stem Cell Transplantation Center

🇺🇸

Seattle, Washington, United States

Primary Children's Medical Center/University of Utah

🇺🇸

Salt Lake City, Utah, United States

Washington University St Louis Children's Hospital

🇺🇸

Saint Louis, Missouri, United States

Texas Children's Hospital

🇺🇸

Houston, Texas, United States

Children's National Medical Center

🇺🇸

Washington, District of Columbia, United States

Hackensack University Medical Center: Department of Pediatrics

🇺🇸

Hackensack, New Jersey, United States

St. Jude Children's Research Hospital

🇺🇸

Memphis, Tennessee, United States

Methodist Children's Hospital of South Texas

🇺🇸

San Antonio, Texas, United States

CHU St. Justine

🇨🇦

Montréal, Quebec, Canada

University of Wisconsin/ American Family Children's Hospital

🇺🇸

Madison, Wisconsin, United States

Medical College of Wisconsin

🇺🇸

Milwaukee, Wisconsin, United States

British Columbia Children's Hospital

🇨🇦

Vancouver, British Columbia, Canada

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