MedPath

Generalized Neonatal Screening of Severe Combined Immunodeficiencies

Not Applicable
Completed
Conditions
Severe Combined Immunodeficiency, Atypical
Interventions
Biological: SCID screening
Registration Number
NCT02244450
Lead Sponsor
Nantes University Hospital
Brief Summary

Severe combined Immunodeficiencies ( SCID ) are a group of inherited diseases of the immune system by characterised profound abnormalities of T cell development . Infants with SCID require prompt clinical response to Prevent life -threatening infection and studies show significantly improved survival in babies Diagnosed at birth as a result of previous family history . SCID follows criteria for population -based newborn screening since it is asymptomatic at birth and fatal within the first year of life, the confirmation of the disease is easy, there is a curative treatment , and it is known that early stem cell transplantation improves survival . Quantification of TRECs (T- cell receptor excision circles ) in DNA extracted from Guthrie samples is a sensitive screening test for Specific and SCID .

The investigators propose in this study to perform a neonatal screening of SCID , in a population of 200,000 babies over a period of two years .

The investigators propose to study the clinical utility and cost effectiveness ratio, and SCID screening to demonstrate that could result in a broad benefit to Individuals detected , making screening relatively cost-effective in spite of the low incidence of the disease .

Detailed Description

The project proposes to study the feasibility and cost-effectiveness ratio ( time management and life expectancy to 10 years) of generalized neonatal screening for SCID children by offering this screening to 200 000 children (100 000 children per year) over the entire territory. Prospective control group consists of children diagnosed with SCID out of 700,000 annual births who do not benefit from screening.

The protocol will be leant against the existing newborn screening , that is to say two more drops of blood are placed on a second Guthrie card when current screening (72 hours of life ) is performed after parents' information and consent. Eleven newborn screening regional associations will be involved with the inclusion of children in about 50 maternity hospitals. The card drawn for the protocol will follow the usual network except that the test for quantifying TRECs will be realized in two laboratories instead of eleven laboratories assigned to RA . Investigative Regional Associations (RAs) represent nearly 600,000 births / year and the amount of 200,000 children will be achieved in two years (duration of inclusion) . All children born in the participating maternity may be included if they meet the inclusion criteria. The result of the screening test for SCID will be available within 21 days after birth, provided that there is no need to request a new sample.

At each of eleven RA is associated a pediatrician referent for immune deficiencies, member of the french reference center (CEREDIH) and who will be responsible to call the parents, offer them a consultation and further exploration if the result of screening is assumed positive.

Analysis of cards from 200,000 children will give the following information:

* Number of children with a presumptive positive screening , requiring a call by the referent pediatrician, consultation and exploration of lymphocyte subpopulations

* Number of children with a negative screening

* Number of children with an inconclusive screening (lack of TRECs and lack of amplification of the reference gene) and requiring a new card,

A micro- costing study will be conducted to assess the cost of testing .

This group of 200,000 children is the experimental group to assess the cost of screening , acceptability by parents (participation rate), the recall rate for abnormal or inconclusive result, the rate of follow-up time for results , the incidence of disease . It will also allow to calculate the specificity of the method .

At the end of the inclusions, the vital status at 18 months with cause of death will be sought for the 200 000 children included , with the CESP ( Centre de Recherche en Epidemiologie et Santé des Populations) via RNIPP (Répertoire National d'Identification des Personnes Physiques) and CepiDc ( Centre d'Epidémiologie sur les causes médicales de décès) . This will establish whether there are SCID in this population which were not detected at birth. Furthermore, the investigators include in the study SCID children diagnosed without screening by pediatricians local referents DIP (including Necker main transplant center) . This will enable to approach the sensitivity of the method . All these data allow the calculation of the predictive values of the test.

In this experimental group will be isolated a group of individuals who screened positive and diagnosed as true SCID . Clinical data for these patients will be collected in an electronic CRF ( CRF ) by the pediatrician referral protocol (Dr Thomas C ) , including:

* The dates and results of explorations : lymphocyte subpopulations , blood count, determination of immunoglobulin levels

* The diagnosis made with identification of the genetic defect

* The date of care before curative treatment ( protected area isolation , anti -infective drugs )

* , Bacterial , fungal anti -viral treatments , and other

* The date of transplant, type of transplant or other treatment ... ...

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
190539
Inclusion Criteria
  • Screening Group: Newborn on day 3 of birth (premature and non premature)
  • Control group: Patients diagnosed with SCID without screening at participating centers
Exclusion Criteria
  • Lack of parental consent
  • Children whose parents are adults under guardianship,
  • Children without health insurance, for the screening group:
  • The early exit of the child from the maternity hospital

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Screened patientsSCID screeningSCID screening: more drops of blood are placed on a second Guthrie card when current screening (72 hours of life ) is performed after parents' information and consent. The card drawn for the protocol will follow the usual network except that the test for quantifying TRECs will be realized to determine the presence of SCID.
Primary Outcome Measures
NameTimeMethod
cost / efficiency ratio of the implementation of the generalized neonatal screening of SCID at birth18 months

Efficacy endpoint: number of children receiving early therapeutic suitable for curative ( transplant, enzyme treatment or gene therapy)

Secondary Outcome Measures
NameTimeMethod
Length of hospitalization of children with SCID in the first 18 months of life18 months
number of avoided deaths18 months
number of patients detected with other T lymphopenia (SCID variants , DiGeorge , severe T lymphopenia non SCID ... )18 months
number of false negative and false positive results18 months

False negative results : patients from the control group diagnosed with SCID without screening who would have a negative screening or patients from the screening group died from a SCID and with a negative screening False positive: patients from the screening group with a positive screening but without SCID

Cost / efficiency ratio of the implementation of the generalized neonatal screening of SCID at birth10 years

Efficacy endpoint: life expectancy of children modeled from the results of the study and data from the literature

The cost of care during the first 18 months of life per child enjoying an early curative treatment in the first 4 months of life.18 months

Costs of care will be estimated during the first 18 months of life of the child.

number of detected SCID patients18 months

Trial Locations

Locations (50)

Paris University Hospital Béclère

🇫🇷

Paris, France

Marseille University Hospital

🇫🇷

Marseille, France

Meaux Hospital

🇫🇷

Meaux, France

Nantes Private Hospital Clinique Jules Verne

🇫🇷

Nantes, France

Libourne Maternity Hospital

🇫🇷

Libourne, France

Marseille University Hospital La Conception

🇫🇷

Marseille, France

Paris University Hospital Bicêtre

🇫🇷

Paris, France

Bordeaux Private Hospital Polyclinique Bordeuax Nord Aquitaine

🇫🇷

Bordeaux, France

Corbeil Essonnes Hospital Sud Francilien

🇫🇷

Corbeil Essonnes, France

Poissy Hospital CHI Poissy-Saint-Germain

🇫🇷

Poissy, France

Rennes University Hospital

🇫🇷

Rennes, France

Paris University Hospital Jean Verdier

🇫🇷

Paris, France

Toulouse University Hospital

🇫🇷

Toulouse, France

Dijon University Hospital

🇫🇷

Dijon, France

Lille University Hospital

🇫🇷

Lille, France

Saint-Herblain Private Hospital Polyclinique de l'Atlantique

🇫🇷

Saint-Herblain, France

Strasbourg University Hospital

🇫🇷

Strasbourg, France

Lyon University Hospital

🇫🇷

Lyon, France

Angers University Hospital

🇫🇷

Angers, France

Montreuil Hospital CHI André Grégroie

🇫🇷

Montreuil, France

Pontoise Hospital René Dubos

🇫🇷

Pontoise, France

Saint-Martin-d'Hère Private Hospital Clinique Belledonne

🇫🇷

Saint-Martin d'Hères, France

Grenoble University Hospital

🇫🇷

Grenoble, France

Angers Private Hospital Clinique de l'Anjou

🇫🇷

Angers, France

Bordeaux Maison de Santé Protestante Bordeaux Bagatelle

🇫🇷

Bordeaux, France

Argenteuil Hospital

🇫🇷

Argenteuil, France

Aulnay-sous-Bois Hospital CHI Robert Ballanger

🇫🇷

Aulnay-sous-Bois, France

Créteil Hospital

🇫🇷

Créteil, France

Bordeaux University Hospital

🇫🇷

Bordeaux, France

Le Blanc Mesnil Private Hospital

🇫🇷

Le Blanc Mesnil, France

Lens Hospital

🇫🇷

Lens, France

Marseille Saint-Joseph Hospital

🇫🇷

Marseille, France

Marseille University Hospital Nord

🇫🇷

Marseille, France

Paris University Hospital Bichat

🇫🇷

Paris, France

Rennes Private Hospital Clinique Mutualiste La Sagesse

🇫🇷

Rennes, France

Roubaix Hospital

🇫🇷

Roubaix, France

Saint-Grégoire Private Hospital

🇫🇷

Rennes, France

Toulouse Private Hospital Clinique Sarrus Teinturiers

🇫🇷

Toulouse, France

Paris University Hospital La Pitié Salpétrière

🇫🇷

Paris, France

Lormont Maternity Hospital Rive Droite

🇫🇷

Lormont, France

Lyon Maternity Hospital

🇫🇷

Lyon, France

Nantes University Hospital

🇫🇷

Nantes, France

Paris University Hospital Armand-Trousseau

🇫🇷

Paris, France

Paris Hospital Saint-Joseph

🇫🇷

Paris, France

Paris Necker University Hospital

🇫🇷

Paris, France

Paris University Hospital Necker

🇫🇷

Paris, France

Paris University Hospital Louis Mourier

🇫🇷

Paris, France

Saint-Mande Army Hospital Begin

🇫🇷

Saint-Mande, France

Strasbourg Private Hospital Clinique Adassa

🇫🇷

Strasbourg, France

Toulouse University Hospital P. DE VIGUIER

🇫🇷

Toulouse, France

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