Association of Physical Activity Level and Sleep Quality with Oxidative Stress and Exercise-related Biomarker Levels in Type 2 Diabetes
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 80
- 试验地点
- 1
- 主要终点
- biomarkers derived from blood sera
研究概览
简要总结
In this study, we investigated the effects of physical activity level and sleep quality level on total antioxidant level (TAS), total oxidant level (TOS), Malondialdehyde (MDA) and Superoxide Dismutase (SOD) which are markers of oxidative stress, irisin, fibronectin type III-protein 5 (FNDC5).
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Prospective
入排标准
- 年龄范围
- 30 Years 至 65 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Being between 30-65 years old
- •Volunteering to participate in the study
- •Being literate
- •Being diagnosed with Type 2 Diabetes
排除标准
- •Having a physical or mental illness that prevents communication
- •malignant disease,
- •infectious disease,
- •chronic obstructive pulmonary disease,
- •liver disease,
- •patients with kidney disease and joint or muscle disease will not be included
结局指标
主要结局
biomarkers derived from blood sera
时间窗: 10 Minutes
blood glucose, total cholesterol, triglycerides, LDL and HDL analyzes
ELISA method of oxidative stress markers
时间窗: 10 minutes
All reagents of the ELISA kits of the oxidative stress markers TAS, TOS, MDA and SOD will be brought to room temperature. Standards will be prepared according to the protocol and added to a 96-well plate with the samples. Buffer solution will be added to each well and incubated for 90 min at room temperature. After each step, necessary washes will be performed and finally chromogenic agents will be added and read spectrophotometrically. Although the timing and additions may vary according to the parameter, in general the procedure is as indicated. Each sample will be run 2 times for both TAS and TOS
Analysis of exercise-related markers by ELISA method
时间窗: 10 minutes
International Physical Activity Questionnaire
时间窗: 5 minutes
Pittsburgh Sleep Quality Index
时间窗: 5 minutes
次要结局
未报告次要终点
研究者
Emine Cihan
Assist.Prof.Dr.
Selcuk University