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Fiber-rich Foods to Treat Obesity and Prevent Colon Cancer

Not Applicable
Active, not recruiting
Conditions
Colon Cancer
Obesity
Interventions
Dietary Supplement: Healthy American Diet
Dietary Supplement: High Fiber Diet Featuring Legumes (HLD)
Registration Number
NCT04780477
Lead Sponsor
Emory University
Brief Summary

This study tests whether a high-fiber diet based on legumes, such as dry beans, can lead to sustained reductions in obesity and colon cancer risk in persons at highest risk, namely overweight or obese, post-polypectomy patients.

Detailed Description

An important knowledge gap concerns the role of fiber in sustaining reduced energy intake to simultaneously manage weight and influence human colorectal cancer risk. Epidemiologic studies have shown an association between a high fiber diet featuring legumes (HLD) and reduced obesity and lower risk for adenoma recurrence or colorectal cancer. There are many plausible mechanisms to explain why high-fiber diets, and especially a HLD, may reduce colorectal cancer risk. First, fiber is fermented by the colonic microbiota to produce short chain fatty acids (SCFA). The SCFA, butyrate, has a remarkable array of colonic mucosal health promoting, anti-inflammatory, and anti-neoplastic properties. Secondly, microbiota break down plant cell walls releasing phytochemicals, which also have powerful anti-inflammatory and anti-carcinogenic effects. Thirdly, colonic transit is accelerated, reducing contact time with luminal carcinogens, such as heterocyclic amines formed from cooked red meat, and secondary bile acids, induced by a high fat diet and synthesized by the colonic microbiota.

Dr. Stephen O'Keefe's lab performed a human randomized controlled crossover feeding study (participants receive both diets) comparing high and low- fiber diets. The study measured mucosal biomarkers of cancer risk (proliferation - % epithelial cells staining positive for Ki67, inflammation - cluster of differentiation 3 (CD3)+ intraepithelial lymphocytes, cluster of differentiation 68 (CD68)+ lamina propria macrophages) made by fecal sampling and colonoscopy. Results suggested that within weeks these markers responded favorably to the high-fiber diet with proliferative rates and inflammatory biomarkers decreasing and microbiota composition adapting to increase butyrogenesis.

The researchers of this study have previously found that fiber may also reduce cancer risk indirectly by promoting weight loss, improving insulin sensitivity and decreasing inflammation. On average, individuals consume a similar weight of food daily; thus, replacing energy dense foods (higher kcal/g, e.g., high fat) with lower energy density foods (lower kcal/g), like legumes, should potentiate weight control. Viscous fiber intake is associated with longer gastric emptying times which over time might contribute to postponing the next eating occasion. Diet may also induce changes in gut microbiome composition leading to negative energy balance. Emerging human evidence links the gut microbiome with insulin resistance, inflammation, and obesity and with adenomatous polyps and colon cancer. In this current study, characterizing gut motility, microbiome, and metabolome composition profiles that may influence weight loss and have a role in the prevention or recurrence of adenomas and colorectal cancer, will provide novel and potentially therapeutic information.

The goal of the research is to conduct a clinical trial featuring study-provided pre-portioned entrées and strategic nutritional instruction to guide participants to integrate legumes into a healthy high-fiber diet pattern. Participants will be provided two entrées per day during months 1 through 3, and one entrée per day during months 4 through 6. Participants will continue their diets during months 7 through 12 but will be responsible for food preparation. The research will target a population at high risk for colorectal cancer, overweight and obese participants with a history of a colon polyp in the past 3 years, to test whether a high-legume, high-fiber diet will simultaneously increase weight loss and suppress intestinal biomarkers of cancer risk compared to a control diet (healthy American). In addition, it will explore potential mechanisms through which the high-legume intervention diet facilitates weight loss and intestinal health.

Recruitment & Eligibility

Status
ACTIVE_NOT_RECRUITING
Sex
All
Target Recruitment
60
Inclusion Criteria
  • free-living adults 40 to 75 years old
  • BMI 25-40 kg/m^2
  • able to provide documentation confirming a colonoscopy within 3 years that found ≥1 adenoma >0.5 cm or a sessile serrated polyp (any)
  • English speaking
  • ambulatory, able to pick up food, participate in clinical exams and laboratory tests
  • able to provide informed consent
Exclusion Criteria
  • serious and/or unstable medical condition as deemed by study physician
  • history of colorectal cancer, bowel resection, polyposis syndrome, or inflammatory bowel disease
  • smoked regularly in the past year
  • dietary restrictions substantially limiting compliance (e.g., must be willing to be randomized to either diet)
  • planning on substantially changing usual exercise behavior in the next 6 months
  • regular or recent use of prescription medication that may alter inflammation markers or gut function as deemed by study physician
  • pregnant women, breast feeding women, or women planning pregnancy within the year of active study participation

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Healthy American Diet Control ArmHealthy American DietParticipants randomized to the healthy American diet control arm will receive pre-portioned meal replacement entrées with legumes replaced by lean chicken or meat.
High Fiber Diet Featuring Legumes (HLD)High Fiber Diet Featuring Legumes (HLD)Participants randomized to the high fiber diet featuring legumes (HLD) will add approximately 30 grams of dietary fiber per day from legume dishes, ensuring a total intake of approximately 50 grams of dietary fiber per day.
Primary Outcome Measures
NameTimeMethod
Change in Body WeightBaseline, Month 6 (end of intense intervention)

Participants will have their body weight measured (in pounds) on a regularly calibrated digital scale while wearing light clothing without shoes.

Change in Ki-67+ LevelBaseline, Month 6 (end of intense intervention)

Colonic mucosal proliferative biomarker Ki-67+ will be measured.

Secondary Outcome Measures
NameTimeMethod
Ki-67+ levelMonth 12

Colonic mucosal proliferative biomarker Ki-67+ will be measured

Body WeightMonth 12

Participants will have their body weight measured (in pounds) on a regularly calibrated digital scale while wearing light clothing without shoes.

Change in Fasting Plasma Glucose LevelBaseline, Month 6, Month 12

Fasting plasma glucose, a biomarker of insulin resistance, is measured by blood test.

Change in Serum C-reactive ProteinBaseline, Month 6, Month 12

Serum C-reactive protein, an indicator of systemic inflammation, is measured by blood test.

Change in CD3+ Intraepithelial Lymphocytes CountBaseline, Month 6, Month 12

CD3+ intraepithelial lymphocytes, a colonic mucosal inflammatory biomarker of colon cancer risk, will be measured by mucosal biopsy.

Change in CD68+ Lamina Propia Macrophages CountBaseline, Month 6, Month 12

CD68+ lamina propia macrophages, a colonic mucosal inflammatory biomarker of colon cancer risk, will be measured by mucosal biopsy.

Change in Gut Transit TimeBaseline, Month 6 (end of intense intervention)

Gut transit time is assessed using an indigestible single-use SmartPill capsule, a receiver, and display software. The SmartPill capsules were discontinued and no longer available after 2023 so participants beginning after January 19, 2024 will not complete this assessment.

Change in Fasting Plasma Insulin LevelBaseline, Month 6, Month 12

Fasting plasma insulin, a biomarker of insulin resistance is measured by blood test.

Trial Locations

Locations (1)

Rollins School of Public Health, Emory University

🇺🇸

Atlanta, Georgia, United States

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